2D Excitation-Emission Fluorescence Mapping Analysis of Plant Food Pigments

Raghavan Chinnambedu Murugesan; Mohammed Thofike Ahmed Choudhury; Alex Rozhin

doi:10.1016/j.foodchem.2023.135875

2D Excitation-Emission Fluorescence Mapping Analysis of Plant Food Pigments

Raghavan Chinnambedu Murugesan, Mohammed Thofike Ahmed Choudhury, Alex Rozhin

Research output: Contribution to journal › Article › peer-review

Abstract

Homogeneous dispersion of plant food pigments is indispensable to study their characteristic fluorescence features for non-destructive rapid monitoring of food systems. However, it is highly challenging to obtain such optical grade homogenized stable dispersion of various plant pigments in aqueous media for tracing their precise fluorescence signatures. Herein, we demonstrate a unique strategy to disperse various pigments, such as chlorophylls, carotenoids and phenolic compounds by the high-speed shear-force mixing of fresh green and red bell peppers (capsicum annuum) in an aqueous medium with followed centrifugation and filtration. An advanced FLuorescence Excitation-emission (FLE) mapping and optical absorption analysis from the optical grade aqueous bell peppers dispersion allow simultaneous probing of chlorophylls, phenolic compounds and carotenoids by their characteristic electronic transitions. The demonstrated sampling protocols and spectroscopic analysis will be highly beneficial to obtain advanced spectroscopic databases from different food materials for rapid food analysis and quality control.

Original language	English
Article number	135875
Number of pages	9
Journal	Food Chemistry
Volume	418
Early online date	6 Mar 2023
DOIs	https://doi.org/10.1016/j.foodchem.2023.135875
Publication status	Published - 30 Aug 2023

Bibliographical note

Copyright © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).

Funding Information: The authors (Raghavan Chinnambedu Murugesan and Alex Rozhin) acknowledge the support of the Marie Skłodowska-Curie Individual Fellowship (MOFUS, # 795356) and of the Wolfson Foundation. Raghavan Chinnambedu Murugesan, Mohammed Thofike Ahmed Choudhury, and Alex Rozhin acknowledge the support of the Enabling Technologies & Innovation Competences Challenge (ETICC) Project, partly funded by the European Regional Development Funds (ERDF).

Keywords

Pigment
chlorophyll
phenolic compounds
carotenoids
absorption
fluorescence

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10.1016/j.foodchem.2023.135875Licence: CC BY 4.0

Chinnambedu Murugesan_Choudhury and Rozhin_2D FLE mapping analysis plant food pigments
Copyright © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
Final published version, 8.7 MBLicence: CC BY 4.0

Cite this

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title = "2D Excitation-Emission Fluorescence Mapping Analysis of Plant Food Pigments",

abstract = "Homogeneous dispersion of plant food pigments is indispensable to study their characteristic fluorescence features for non-destructive rapid monitoring of food systems. However, it is highly challenging to obtain such optical grade homogenized stable dispersion of various plant pigments in aqueous media for tracing their precise fluorescence signatures. Herein, we demonstrate a unique strategy to disperse various pigments, such as chlorophylls, carotenoids and phenolic compounds by the high-speed shear-force mixing of fresh green and red bell peppers (capsicum annuum) in an aqueous medium with followed centrifugation and filtration. An advanced FLuorescence Excitation-emission (FLE) mapping and optical absorption analysis from the optical grade aqueous bell peppers dispersion allow simultaneous probing of chlorophylls, phenolic compounds and carotenoids by their characteristic electronic transitions. The demonstrated sampling protocols and spectroscopic analysis will be highly beneficial to obtain advanced spectroscopic databases from different food materials for rapid food analysis and quality control.",

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author = "{Chinnambedu Murugesan}, Raghavan and Choudhury, {Mohammed Thofike Ahmed} and Alex Rozhin",

note = "Copyright {\textcopyright} 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/). Funding Information: The authors (Raghavan Chinnambedu Murugesan and Alex Rozhin) acknowledge the support of the Marie Sk{\l}odowska-Curie Individual Fellowship (MOFUS, # 795356) and of the Wolfson Foundation. Raghavan Chinnambedu Murugesan, Mohammed Thofike Ahmed Choudhury, and Alex Rozhin acknowledge the support of the Enabling Technologies & Innovation Competences Challenge (ETICC) Project, partly funded by the European Regional Development Funds (ERDF). ",

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T1 - 2D Excitation-Emission Fluorescence Mapping Analysis of Plant Food Pigments

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AU - Choudhury, Mohammed Thofike Ahmed

AU - Rozhin, Alex

N1 - Copyright © 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/). Funding Information: The authors (Raghavan Chinnambedu Murugesan and Alex Rozhin) acknowledge the support of the Marie Skłodowska-Curie Individual Fellowship (MOFUS, # 795356) and of the Wolfson Foundation. Raghavan Chinnambedu Murugesan, Mohammed Thofike Ahmed Choudhury, and Alex Rozhin acknowledge the support of the Enabling Technologies & Innovation Competences Challenge (ETICC) Project, partly funded by the European Regional Development Funds (ERDF).

PY - 2023/8/30

Y1 - 2023/8/30

N2 - Homogeneous dispersion of plant food pigments is indispensable to study their characteristic fluorescence features for non-destructive rapid monitoring of food systems. However, it is highly challenging to obtain such optical grade homogenized stable dispersion of various plant pigments in aqueous media for tracing their precise fluorescence signatures. Herein, we demonstrate a unique strategy to disperse various pigments, such as chlorophylls, carotenoids and phenolic compounds by the high-speed shear-force mixing of fresh green and red bell peppers (capsicum annuum) in an aqueous medium with followed centrifugation and filtration. An advanced FLuorescence Excitation-emission (FLE) mapping and optical absorption analysis from the optical grade aqueous bell peppers dispersion allow simultaneous probing of chlorophylls, phenolic compounds and carotenoids by their characteristic electronic transitions. The demonstrated sampling protocols and spectroscopic analysis will be highly beneficial to obtain advanced spectroscopic databases from different food materials for rapid food analysis and quality control.

AB - Homogeneous dispersion of plant food pigments is indispensable to study their characteristic fluorescence features for non-destructive rapid monitoring of food systems. However, it is highly challenging to obtain such optical grade homogenized stable dispersion of various plant pigments in aqueous media for tracing their precise fluorescence signatures. Herein, we demonstrate a unique strategy to disperse various pigments, such as chlorophylls, carotenoids and phenolic compounds by the high-speed shear-force mixing of fresh green and red bell peppers (capsicum annuum) in an aqueous medium with followed centrifugation and filtration. An advanced FLuorescence Excitation-emission (FLE) mapping and optical absorption analysis from the optical grade aqueous bell peppers dispersion allow simultaneous probing of chlorophylls, phenolic compounds and carotenoids by their characteristic electronic transitions. The demonstrated sampling protocols and spectroscopic analysis will be highly beneficial to obtain advanced spectroscopic databases from different food materials for rapid food analysis and quality control.

KW - Pigment

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KW - phenolic compounds

KW - carotenoids

KW - absorption

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U2 - 10.1016/j.foodchem.2023.135875

DO - 10.1016/j.foodchem.2023.135875

M3 - Article

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VL - 418

JO - Food Chemistry

JF - Food Chemistry

M1 - 135875

ER -

2D Excitation-Emission Fluorescence Mapping Analysis of Plant Food Pigments

Abstract

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Keywords

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