Abstract
Due to the complexity of the structure of the tooth periodontium, regeneration of the full tooth attachment is not a trivial task. There is also a gap in models that can represent human tooth attachment in vitro and in vivo.
Aim of the study: to develop a bilayered in vitro construct that simulates the tooth periodontium, for the purpose of tissue regeneration and investigation of physiologic and orthodontic loading.
Methods: Two types of materials were used to develop this construct: Sol-Gel 60S10Mg scaffold (University of Erlangen) representing the hard tissue component of the and commercially available Geistlich Bio-Gide® collagen membrane representing the soft tissue component of the tooth attachment. Each scaffold was dynamically seeded with periodontal ligament cells. The seeded scaffolds were either cultured separately, or combined in a bilayered construct, for 2 weeks. Characterization of the individual scaffolds and the bilayered constructs included biological characterization: (cell viability, SEM imaging to confirm cell attachment and viability qRT-PCR expression for periodontium regeneration markers), and mechanical characterisation of scaffolds and constructs.
Results: HPDLCs enjoyed a biocompatible 3D environment within the bi-layered construct components as demonstrated by live/dead images and LDH assay. Gene expression showed variation between individual scaffolds and constructs detached from the bilayer. Most genes showed a drop in expression in the construct except for markers of angiogenesis which showed their highest expression in Bio-Gide® detached constructs.
Conclusion and clinical significance: the development of this model is important for physiologic simulation invitro and for tissue regeneration purposes of the tooth periodontium.
Original language | English |
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Pages (from-to) | 232-245 |
Number of pages | 14 |
Journal | European Cells and Materials |
Volume | 42 |
DOIs | |
Publication status | Published - 11 Oct 2021 |
Bibliographical note
This article is distributed in accordance with Creative Commons Attribution Licence(http://creativecommons.org/licenses/by-sa/4.0/).
Keywords
- periodontal regeneration
- physiologic simulation
- in-vitro models
- bilayered construct
- tissue engineering
- periodontal ligament cells