A Robust Method for Adjustment of Laser Speckle Contrast Imaging during Transcranial Mouse Brain Visualization

Vyacheslav Kalchenko, Anton Sdobnov, Igor Meglinski, Yuri Kuznetsov, Guillaume Molodij, Alon Harmelin

Research output: Contribution to journalLetter, comment/opinion or interviewpeer-review


Laser speckle imaging (LSI) is a well-known and useful approach for the non-invasive visualization of flows and microcirculation localized in turbid scattering media, including biological tissues (such as brain vasculature, skin capillaries etc.). Despite an extensive use of LSI for brain imaging, the LSI technique has several critical limitations. One of them is associated with inability to resolve a functionality of vessels. This limitation also leads to the systematic error in the quantitative interpretation of values of speckle contrast obtained for different vessel types, such as sagittal sinus, arteries, and veins. Here, utilizing a combined use of LSI and fluorescent intravital microscopy (FIM), we present a simple and robust method to overcome the limitations mentioned above for the LSI approach. The proposed technique provides more relevant, abundant, and valuable information regarding perfusion rate ration between different types of vessels that makes this method highly useful for in vivo brain surgical operations.

Original languageEnglish
Article number80
Issue number3
Publication statusPublished - 13 Jul 2019

Bibliographical note

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access
article distributed under the terms and conditions of the Creative Commons Attribution
(CC BY) license (http://creativecommons.org/licenses/by/4.0/).

Funding: The Henry Chanoch Krenter Institute for Biomedical Imaging and Genomics (Sta Scientists grant program), Academy of Finland (project 326204), NATO (project SPS G5147), MEPhI Academic Excellence Project (Contract No. 02.a03.21.0005), National Research Tomsk State University Academic D.I. Mendeleev Fund Program, Finnish Cultural Foundation (00180998) grant.


  • Cerebral blood vessels
  • In vivo fluorescence imaging
  • Middle cerebral artery
  • Non-invasive optical imaging
  • Speckle contrast
  • Transcranial imaging


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