Activation and repression at the Escherichia coli ynfEFGHI operon promoter

Meng Xu; Stephen J W Busby; Douglas F Browning

doi:10.1128/JB.00040-09

Activation and repression at the Escherichia coli ynfEFGHI operon promoter

Meng Xu, Stephen J W Busby, Douglas F Browning

Research output: Contribution to journal › Article › peer-review

Abstract

Induction of the Escherichia coli K-12 ynfEFGHI operon in response to anaerobiosis is repressed by nitrate ions. In this study, we show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. Electromobility shift assays show that NarL does not prevent RNA polymerase binding and suggest that repression may involve a quaternary NarL-FNR-RNA polymerase-promoter complex.

Original language	English
Pages (from-to)	3172-3176
Number of pages	5
Journal	Journal of Bacteriology
Volume	191
Issue number	9
DOIs	https://doi.org/10.1128/JB.00040-09
Publication status	Published - May 2009

Keywords

Anaerobiosis
Base Sequence
DNA-Binding Proteins/physiology
DNA-Directed RNA Polymerases/metabolism
Electrophoretic Mobility Shift Assay
Escherichia coli K12/genetics
Escherichia coli Proteins/physiology
Gene Expression Regulation, Bacterial
Iron-Sulfur Proteins/physiology
Molecular Sequence Data
Operon
Promoter Regions, Genetic
Protein Binding
Transcriptional Activation

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10.1128/JB.00040-09

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title = "Activation and repression at the Escherichia coli ynfEFGHI operon promoter",

abstract = "Induction of the Escherichia coli K-12 ynfEFGHI operon in response to anaerobiosis is repressed by nitrate ions. In this study, we show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. Electromobility shift assays show that NarL does not prevent RNA polymerase binding and suggest that repression may involve a quaternary NarL-FNR-RNA polymerase-promoter complex.",

keywords = "Anaerobiosis, Base Sequence, DNA-Binding Proteins/physiology, DNA-Directed RNA Polymerases/metabolism, Electrophoretic Mobility Shift Assay, Escherichia coli K12/genetics, Escherichia coli Proteins/physiology, Gene Expression Regulation, Bacterial, Iron-Sulfur Proteins/physiology, Molecular Sequence Data, Operon, Promoter Regions, Genetic, Protein Binding, Transcriptional Activation",

author = "Meng Xu and Busby, {Stephen J W} and Browning, {Douglas F}",

year = "2009",

month = may,

doi = "10.1128/JB.00040-09",

language = "English",

volume = "191",

pages = "3172--3176",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "9",

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TY - JOUR

T1 - Activation and repression at the Escherichia coli ynfEFGHI operon promoter

AU - Xu, Meng

AU - Busby, Stephen J W

AU - Browning, Douglas F

PY - 2009/5

Y1 - 2009/5

N2 - Induction of the Escherichia coli K-12 ynfEFGHI operon in response to anaerobiosis is repressed by nitrate ions. In this study, we show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. Electromobility shift assays show that NarL does not prevent RNA polymerase binding and suggest that repression may involve a quaternary NarL-FNR-RNA polymerase-promoter complex.

AB - Induction of the Escherichia coli K-12 ynfEFGHI operon in response to anaerobiosis is repressed by nitrate ions. In this study, we show that the global transcription factor FNR is a class II activator at the ynfEFGHI promoter and that NarL represses activation by binding to a single target that overlaps the promoter -10 element. Electromobility shift assays show that NarL does not prevent RNA polymerase binding and suggest that repression may involve a quaternary NarL-FNR-RNA polymerase-promoter complex.

KW - Anaerobiosis

KW - Base Sequence

KW - DNA-Binding Proteins/physiology

KW - DNA-Directed RNA Polymerases/metabolism

KW - Electrophoretic Mobility Shift Assay

KW - Escherichia coli K12/genetics

KW - Escherichia coli Proteins/physiology

KW - Gene Expression Regulation, Bacterial

KW - Iron-Sulfur Proteins/physiology

KW - Molecular Sequence Data

KW - Operon

KW - Promoter Regions, Genetic

KW - Protein Binding

KW - Transcriptional Activation

UR - https://journals.asm.org/doi/10.1128/JB.00040-09

U2 - 10.1128/JB.00040-09

DO - 10.1128/JB.00040-09

M3 - Article

C2 - 19251855

SN - 0021-9193

VL - 191

SP - 3172

EP - 3176

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 9

ER -

Activation and repression at the Escherichia coli ynfEFGHI operon promoter

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