Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD

A.L. Perry, Tony Worthington, Anthony Hilton, Peter A. Lambert*, A.J. Stirling, T.S.J. Elliott

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists. © 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)51-55
Number of pages5
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - 7 Nov 2003


  • molecular typing
  • propionibacterium acnes
  • random amplification of polymorphic DNA


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