TY - JOUR
T1 - Analysis of plasmid profiles of Aeromonas salmonicida isolates by pulsed field gel electrophoresis
AU - Livesley, M. Anne
AU - Smith, Stephen N.
AU - Armstrong, Richard A.
AU - Barker, Gavin A.
PY - 1997/1/15
Y1 - 1997/1/15
N2 - Plasmid constitutions of Aeromonas salmonicida isolates were characterised by flat-bed and pulsed field gel electrophoresis. Resolution of plasmids by pulsed field gel electrophoresis was greater and more consistent than that achieved by flat-bed gel electrophoresis. The number of plasmids separated by pulsed field gel electrophoresis varied between A. salmonicida isolates, with five being the most common number present in the isolates used in this study. Plasmid profiles were diverse and the reproducibility of the distances migrated facilitated the use of principal components analysis for the characterisation of the isolates. Isolates were grouped according to the number of plasmids supported. Further principal components analysis of groups of isolates supporting five and seven plasmids showed a spatial separation of plasmids based upon distance migrated. Principal components analysis of plasmid profiles and antimicrobial minimum inhibitory concentrations could not be correlated suggesting that resistance to antimicrobial agents is not associated with either one plasmid or a particular plasmid constitution.
AB - Plasmid constitutions of Aeromonas salmonicida isolates were characterised by flat-bed and pulsed field gel electrophoresis. Resolution of plasmids by pulsed field gel electrophoresis was greater and more consistent than that achieved by flat-bed gel electrophoresis. The number of plasmids separated by pulsed field gel electrophoresis varied between A. salmonicida isolates, with five being the most common number present in the isolates used in this study. Plasmid profiles were diverse and the reproducibility of the distances migrated facilitated the use of principal components analysis for the characterisation of the isolates. Isolates were grouped according to the number of plasmids supported. Further principal components analysis of groups of isolates supporting five and seven plasmids showed a spatial separation of plasmids based upon distance migrated. Principal components analysis of plasmid profiles and antimicrobial minimum inhibitory concentrations could not be correlated suggesting that resistance to antimicrobial agents is not associated with either one plasmid or a particular plasmid constitution.
KW - aeromonas salmonicida
KW - plasmid
KW - principal components analysis
KW - pulsed field gel electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=0031034202&partnerID=8YFLogxK
UR - http://www3.interscience.wiley.com/journal/119173202/abstract
U2 - 10.1016/S0378-1097(96)00492-2
DO - 10.1016/S0378-1097(96)00492-2
M3 - Article
SN - 0378-1097
VL - 146
SP - 297
EP - 301
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 2
ER -