Abstract
We utilize Laser Speckle Contrast Imaging (LSCI) for visualizing cerebral blood flow in mice during and post-cardiac arrest. Analyzing LSCI images, we noted temporal blood flow variations across the brain surface for hours postmortem. Fast Fourier Transform (FFT) analysis depicted blood flow and microcirculation decay post-death. Continuous Wavelet Transform (CWT) identified potential cerebral hemodynamic synchronization patterns. Additionally, non-negative matrix factorization (NMF) with four components segmented LSCI images, revealing structural subcomponent alterations over time. This integrated approach of LSCI, FFT, CWT, and NMF offers a comprehensive tool for studying cerebral blood flow dynamics, metaphorically capturing the ‘end of the tunnel’ experience. Results showed primary postmortem hemodynamic activity in the olfactory bulbs, followed by blood microflow relocations between somatosensory and visual cortical regions via the superior sagittal sinus. This method opens new avenues for exploring these phenomena, potentially linking neuroscientific insights with mysteries surrounding consciousness and perception at life's end.
| Original language | English |
|---|---|
| Article number | e202400017 |
| Number of pages | 11 |
| Journal | Journal of Biophotonics |
| Volume | 17 |
| Issue number | 7 |
| Early online date | 7 May 2024 |
| DOIs | |
| Publication status | Published - Jul 2024 |
Bibliographical note
Copyright © 2024 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.Data Access Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.Keywords
- blood flow
- brain microcirculation
- continuous wavelet transform
- laser speckle contrast
- non-negative matrix factorization
- postmortem