TY - JOUR
T1 - Brain metabolism changes in cases of impaired breathing or blood circulation in rodents evaluated by real time optical spectroscopy methods
AU - Piavchenko, Gennadii A.
AU - Seryogina, Evgeniya S.
AU - Kandurova, Ksenia Yu
AU - Shupletsov, Valery V.
AU - Kozlov, Igor O.
AU - Stavtsev, Dmitry D.
AU - Stelmashchuk, Olga A.
AU - Zherebtsov, Evgeny A.
AU - Dremin, Viktor V.
AU - Alekseyev, Aleksander G.
AU - Kuznetsov, Sergey L.
AU - Dunaev, Andrey V.
AU - Meglinski, Igor V.
N1 - Copyright 2020 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/2/21
Y1 - 2020/2/21
N2 - The aim of the study was to compare the metabolic activity of brain cortex after the acute hypoxia caused by the impairment of breathing or blood circulation. Male Wistar rats were randomized in two groups: impaired breathing and blood circulation failure groups. Fluorescence under 365 and 450 nm excitation and diffuse reflectance intensity at 550-820 nm range were estimated. We found that after long-term hypoxic conditions, notable metabolic changes occur. We suppose that oxygen deficiency causes an activation of the GABA shunt mechanism. In cases of blood circulation failure, fluorescence intensity changes faster than in cases of breathing impairment.
AB - The aim of the study was to compare the metabolic activity of brain cortex after the acute hypoxia caused by the impairment of breathing or blood circulation. Male Wistar rats were randomized in two groups: impaired breathing and blood circulation failure groups. Fluorescence under 365 and 450 nm excitation and diffuse reflectance intensity at 550-820 nm range were estimated. We found that after long-term hypoxic conditions, notable metabolic changes occur. We suppose that oxygen deficiency causes an activation of the GABA shunt mechanism. In cases of blood circulation failure, fluorescence intensity changes faster than in cases of breathing impairment.
KW - Brain metabolism
KW - Diffuse reflectance spectra
KW - FAD
KW - Fluorescence spectroscopy
KW - Laser speckle contrast imaging
KW - NADH
KW - Neuronal hypoxia
KW - Resuscitation procedures
UR - http://www.scopus.com/inward/record.url?scp=85082726209&partnerID=8YFLogxK
UR - https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11234/2545708/Brain-metabolism-changes-in-cases-of-impaired-breathing-or-blood/10.1117/12.2545708.short
U2 - 10.1117/12.2545708
DO - 10.1117/12.2545708
M3 - Conference article
AN - SCOPUS:85082726209
SN - 1605-7422
VL - 11234
JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
M1 - 112341E
T2 - Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis 2020
Y2 - 2 February 2020 through 5 February 2020
ER -