Chromatography of oxysterols

Irundika H.k. Dias; Steven R. Wilson; Hanne Roberg-larsen

doi:10.1016/j.biochi.2018.05.004

Chromatography of oxysterols

Irundika H.k. Dias, Steven R. Wilson, Hanne Roberg-larsen

Research output: Contribution to journal › Review article › peer-review

Abstract

Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.

Original language	English
Pages (from-to)	3-12
Number of pages	10
Journal	Biochimie
Volume	153
Early online date	9 May 2018
DOIs	https://doi.org/10.1016/j.biochi.2018.05.004
Publication status	Published - Oct 2018

Bibliographical note

© 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Funding: Kidney Research Foundation PDF3/2014 and Alzheimer's Research UK network grants.

Keywords

(max 6)Oxysterols
Chromatography
Separation
Lipids
Sterols

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10.1016/j.biochi.2018.05.004

CHROMATOGRAPHY OF OXYSTEROLS
© 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Accepted author manuscript, 1.96 MBLicence: CC BY-NC-ND 3.0

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title = "Chromatography of oxysterols",

abstract = "Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.",

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doi = "10.1016/j.biochi.2018.05.004",

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AU - Dias, Irundika H.k.

AU - Wilson, Steven R.

AU - Roberg-larsen, Hanne

N1 - © 2018, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International Funding: Kidney Research Foundation PDF3/2014 and Alzheimer's Research UK network grants.

PY - 2018/10

Y1 - 2018/10

N2 - Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.

AB - Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. We will present the role of stationary phases, mobile phases, and dimensions and geometries of particles/columns. We discuss how these parameters may affect the chromatography, regarding factors such as speed and resolution. Finally, we present some less explored avenues for separation of oxysterols.

KW - (max 6)Oxysterols

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KW - Separation

KW - Lipids

KW - Sterols

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U2 - 10.1016/j.biochi.2018.05.004

DO - 10.1016/j.biochi.2018.05.004

M3 - Review article

SN - 0300-9084

VL - 153

SP - 3

EP - 12

JO - Biochimie

JF - Biochimie

ER -

Chromatography of oxysterols

Abstract

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Keywords

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