Combined laser speckle imaging and fluorescent intravital microscopy for monitoring acute vascular permeability reaction

Vyacheslav Kalchenko, Igor Meglinski, Anton Sdobnov, Yuri Kuznetsov, Alon Harmelin

Research output: Contribution to journalArticlepeer-review


Optical clearing agents (OCAs) and many chemicals are widely used in functional diagnosis of skin tissues. Numerous studies are associated with the transcutaneous diffusion of OCA in epidermal, dermal, and hypodermal tissues, which results in changing their optical properties. In addition, an objective approach that is suitable for screening the influence of utilized OCA, as well as various chemical agents, synthetics, and nanomaterials, on blood and lymph flows is highly desirable. In our study, a highly sensitive laser speckle imaging (LSI) system and fluorescent intravital microscopy (FIM) were used team-wise to inspect the acute skin vascular permeability reaction in mouse ear during the local application of OCA on the skin surface. Fluorescent contrast material administrated intravenously was used for quantitatively assessing the intensity of vascular permeability reaction and the strength of skin irritation. The obtained results suggest that a combined use of LSI and FIM is highly effective for monitoring the cutaneous vascular permeability reaction, with great potential for assessment of allergic reactions of skin in response to interactions with chemical substances.

Original languageEnglish
Article number060501
Pages (from-to)1-4
Number of pages4
JournalJournal of Biomedical Optics
Issue number6
Publication statusPublished - 31 May 2019

Bibliographical note

© The Authors. Published by SPIE under a Creative Commons Attribution 4.0
Unported License. Distribution or reproduction of this work in whole or in
part requires full attribution of the original publication, including its DOI.


  • acute vascular reaction
  • allergens
  • contact irritant
  • fluorescence
  • laser speckle imaging
  • optical clearing
  • skin
  • vascular permeability


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