Complex Measurements of Fluorescence and Speckle Contrast in Laboratory Mice during Pancreas Ischemia Modeling

Valery Shupletsov; Ksenia Kandurova; Evgeniya Seryogina; Gennadii Piavchenko; Viktor Dremin; Andrian Mamoshin; Andrey Dunaev

Complex Measurements of Fluorescence and Speckle Contrast in Laboratory Mice during Pancreas Ischemia Modeling

Valery Shupletsov, Ksenia Kandurova, Evgeniya Seryogina, Gennadii Piavchenko, Viktor Dremin, Andrian Mamoshin, Andrey Dunaev

Aston Institute of Photonic Technologies (AiPT)

Research output: Contribution to journal › Conference article › peer-review

Abstract

The paper describes the experiment at pancreas ischemia model carried out by combining the methods of fluorescence spectroscopy and laser speckle contrast imaging in laboratory mice. The measurement was performed before and after ischemia to evaluate the relationship between the changes of blood flow and metabolic activity. The results showed high positive correlation between fluorescence intensity at excitation wavelength of 365 nm and speckle contrast. At the same time, 450 nm fluorescence intensity and speckle contrast showed high negative correlation as well as two kinds of fluorescence maxima. The proposed approach seems promising for further studies and future clinical testing.

Original language	English
Article number	1114001-212
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	11140
Publication status	Published - 21 Apr 2019
Event	Biomedical Imaging and Sensing Conference 2019, BISC 2019 - Yokohama, Japan Duration: 22 Apr 2019 → 26 Apr 2019

Keywords

Fluorescence spectroscopy
Ischemia
Mice
Pancreas
Speckle contrast

Cite this

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title = "Complex Measurements of Fluorescence and Speckle Contrast in Laboratory Mice during Pancreas Ischemia Modeling",

abstract = "The paper describes the experiment at pancreas ischemia model carried out by combining the methods of fluorescence spectroscopy and laser speckle contrast imaging in laboratory mice. The measurement was performed before and after ischemia to evaluate the relationship between the changes of blood flow and metabolic activity. The results showed high positive correlation between fluorescence intensity at excitation wavelength of 365 nm and speckle contrast. At the same time, 450 nm fluorescence intensity and speckle contrast showed high negative correlation as well as two kinds of fluorescence maxima. The proposed approach seems promising for further studies and future clinical testing.",

keywords = "Fluorescence spectroscopy, Ischemia, Mice, Pancreas, Speckle contrast",

author = "Valery Shupletsov and Ksenia Kandurova and Evgeniya Seryogina and Gennadii Piavchenko and Viktor Dremin and Andrian Mamoshin and Andrey Dunaev",

year = "2019",

month = apr,

day = "21",

language = "English",

volume = "11140",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Biomedical Imaging and Sensing Conference 2019, BISC 2019 ; Conference date: 22-04-2019 Through 26-04-2019",

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Complex Measurements of Fluorescence and Speckle Contrast in Laboratory Mice during Pancreas Ischemia Modeling. / Shupletsov, Valery; Kandurova, Ksenia; Seryogina, Evgeniya et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 11140, 1114001-212, 21.04.2019.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Complex Measurements of Fluorescence and Speckle Contrast in Laboratory Mice during Pancreas Ischemia Modeling

AU - Shupletsov, Valery

AU - Kandurova, Ksenia

AU - Seryogina, Evgeniya

AU - Piavchenko, Gennadii

AU - Dremin, Viktor

AU - Mamoshin, Andrian

AU - Dunaev, Andrey

PY - 2019/4/21

Y1 - 2019/4/21

N2 - The paper describes the experiment at pancreas ischemia model carried out by combining the methods of fluorescence spectroscopy and laser speckle contrast imaging in laboratory mice. The measurement was performed before and after ischemia to evaluate the relationship between the changes of blood flow and metabolic activity. The results showed high positive correlation between fluorescence intensity at excitation wavelength of 365 nm and speckle contrast. At the same time, 450 nm fluorescence intensity and speckle contrast showed high negative correlation as well as two kinds of fluorescence maxima. The proposed approach seems promising for further studies and future clinical testing.

AB - The paper describes the experiment at pancreas ischemia model carried out by combining the methods of fluorescence spectroscopy and laser speckle contrast imaging in laboratory mice. The measurement was performed before and after ischemia to evaluate the relationship between the changes of blood flow and metabolic activity. The results showed high positive correlation between fluorescence intensity at excitation wavelength of 365 nm and speckle contrast. At the same time, 450 nm fluorescence intensity and speckle contrast showed high negative correlation as well as two kinds of fluorescence maxima. The proposed approach seems promising for further studies and future clinical testing.

KW - Fluorescence spectroscopy

KW - Ischemia

KW - Mice

KW - Pancreas

KW - Speckle contrast

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UR - https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11140.toc

M3 - Conference article

AN - SCOPUS:85065713849

SN - 0277-786X

VL - 11140

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

M1 - 1114001-212

T2 - Biomedical Imaging and Sensing Conference 2019, BISC 2019

Y2 - 22 April 2019 through 26 April 2019

ER -

Complex Measurements of Fluorescence and Speckle Contrast in Laboratory Mice during Pancreas Ischemia Modeling

Abstract

Keywords

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