Direct inhibitory effects of an antisense oligodeoxynucleotide upon the volume-sensitive chloride current in rat osteoblast-like (ROS 17/2.8) cells

Martin Gosling; Saghir Akhtar; J.W. Smith; David R. Poyner

doi:10.1006/bbrc.1996.1460

Direct inhibitory effects of an antisense oligodeoxynucleotide upon the volume-sensitive chloride current in rat osteoblast-like (ROS 17/2.8) cells

Martin Gosling, Saghir Akhtar, J.W. Smith, David R. Poyner

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of a 15-mer antisense c-myc phosphorothioate modified oligodeoxynucleotide (OdN) upon the volume-sensitive Cl- current in ROS 17/2.8 cells were investigated using the whole-cell configuration of the patch clamp technique. At 5 microM, the OdN reversibly inhibited the current in a voltage- and time-dependent fashion. This was evident from the reduction in the peak current as assessed at the termination of each voltage pulse and an acceleration of the time-dependent inactivation present at strongly depolarised potentials. The kinetic modifications induced by the OdN suggest it may act by blocking the pore of open channels when the cell membrane potential is depolarised.

Original language	English
Pages (from-to)	15-19
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	227
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1996.1460
Publication status	Published - 3 Oct 1996

Keywords

animals
cell
line
chlorides
membrane
potentials
oligonucleotides
antisense osmolar concentration osteoblasts
proto-oncogene proteins
c-myc
rats

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10.1006/bbrc.1996.1460

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title = "Direct inhibitory effects of an antisense oligodeoxynucleotide upon the volume-sensitive chloride current in rat osteoblast-like (ROS 17/2.8) cells",

abstract = "The effects of a 15-mer antisense c-myc phosphorothioate modified oligodeoxynucleotide (OdN) upon the volume-sensitive Cl- current in ROS 17/2.8 cells were investigated using the whole-cell configuration of the patch clamp technique. At 5 microM, the OdN reversibly inhibited the current in a voltage- and time-dependent fashion. This was evident from the reduction in the peak current as assessed at the termination of each voltage pulse and an acceleration of the time-dependent inactivation present at strongly depolarised potentials. The kinetic modifications induced by the OdN suggest it may act by blocking the pore of open channels when the cell membrane potential is depolarised.",

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AU - Gosling, Martin

AU - Akhtar, Saghir

AU - Smith, J.W.

AU - Poyner, David R.

PY - 1996/10/3

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N2 - The effects of a 15-mer antisense c-myc phosphorothioate modified oligodeoxynucleotide (OdN) upon the volume-sensitive Cl- current in ROS 17/2.8 cells were investigated using the whole-cell configuration of the patch clamp technique. At 5 microM, the OdN reversibly inhibited the current in a voltage- and time-dependent fashion. This was evident from the reduction in the peak current as assessed at the termination of each voltage pulse and an acceleration of the time-dependent inactivation present at strongly depolarised potentials. The kinetic modifications induced by the OdN suggest it may act by blocking the pore of open channels when the cell membrane potential is depolarised.

AB - The effects of a 15-mer antisense c-myc phosphorothioate modified oligodeoxynucleotide (OdN) upon the volume-sensitive Cl- current in ROS 17/2.8 cells were investigated using the whole-cell configuration of the patch clamp technique. At 5 microM, the OdN reversibly inhibited the current in a voltage- and time-dependent fashion. This was evident from the reduction in the peak current as assessed at the termination of each voltage pulse and an acceleration of the time-dependent inactivation present at strongly depolarised potentials. The kinetic modifications induced by the OdN suggest it may act by blocking the pore of open channels when the cell membrane potential is depolarised.

KW - animals

KW - cell

KW - line

KW - chlorides

KW - membrane

KW - potentials

KW - oligonucleotides

KW - antisense osmolar concentration osteoblasts

KW - proto-oncogene proteins

KW - c-myc

KW - rats

UR - https://www.sciencedirect.com/science/article/pii/S0006291X96914606?via%3Dihub

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DO - 10.1006/bbrc.1996.1460

M3 - Article

SN - 0006-291X

VL - 227

SP - 15

EP - 19

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Direct inhibitory effects of an antisense oligodeoxynucleotide upon the volume-sensitive chloride current in rat osteoblast-like (ROS 17/2.8) cells

Abstract

Keywords

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