Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site

Douglas F Browning; David J Lee; Stephen Spiro; Stephen J W Busby

doi:10.1128/JB.00218-10

Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site

Douglas F Browning, David J Lee, Stephen Spiro, Stephen J W Busby

Research output: Contribution to journal › Article › peer-review

Abstract

FNR-dependent activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF, which bind to overlapping targets adjacent to the DNA site for FNR. The NsrR target is inactivated by mutation at the Salmonella enterica serovar Typhimurium nrf promoter.

Original language	English
Pages (from-to)	3824-3828
Number of pages	5
Journal	Journal of Bacteriology
Volume	192
Issue number	14
DOIs	https://doi.org/10.1128/JB.00218-10
Publication status	Published - Jul 2010

Keywords

Base Sequence
Cytochrome c Group/genetics
DNA, Bacterial
DNA-Binding Proteins/genetics
Down-Regulation
Escherichia coli K12/genetics
Escherichia coli Proteins/genetics
Gene Expression Regulation, Bacterial/physiology
Mutation
Nitric Oxide/metabolism
Promoter Regions, Genetic/physiology
Salmonella typhimurium/genetics
Transcription Factors/genetics
Transcription, Genetic

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10.1128/JB.00218-10

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title = "Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site",

abstract = "FNR-dependent activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF, which bind to overlapping targets adjacent to the DNA site for FNR. The NsrR target is inactivated by mutation at the Salmonella enterica serovar Typhimurium nrf promoter.",

keywords = "Base Sequence, Cytochrome c Group/genetics, DNA, Bacterial, DNA-Binding Proteins/genetics, Down-Regulation, Escherichia coli K12/genetics, Escherichia coli Proteins/genetics, Gene Expression Regulation, Bacterial/physiology, Mutation, Nitric Oxide/metabolism, Promoter Regions, Genetic/physiology, Salmonella typhimurium/genetics, Transcription Factors/genetics, Transcription, Genetic",

author = "Browning, {Douglas F} and Lee, {David J} and Stephen Spiro and Busby, {Stephen J W}",

year = "2010",

month = jul,

doi = "10.1128/JB.00218-10",

language = "English",

volume = "192",

pages = "3824--3828",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "14",

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TY - JOUR

T1 - Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site

AU - Browning, Douglas F

AU - Lee, David J

AU - Spiro, Stephen

AU - Busby, Stephen J W

PY - 2010/7

Y1 - 2010/7

N2 - FNR-dependent activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF, which bind to overlapping targets adjacent to the DNA site for FNR. The NsrR target is inactivated by mutation at the Salmonella enterica serovar Typhimurium nrf promoter.

AB - FNR-dependent activation of the Escherichia coli K-12 nrf promoter is downregulated by the nitric oxide-sensitive NsrR protein together with the nucleoid-associated protein IHF, which bind to overlapping targets adjacent to the DNA site for FNR. The NsrR target is inactivated by mutation at the Salmonella enterica serovar Typhimurium nrf promoter.

KW - Base Sequence

KW - Cytochrome c Group/genetics

KW - DNA, Bacterial

KW - DNA-Binding Proteins/genetics

KW - Down-Regulation

KW - Escherichia coli K12/genetics

KW - Escherichia coli Proteins/genetics

KW - Gene Expression Regulation, Bacterial/physiology

KW - Mutation

KW - Nitric Oxide/metabolism

KW - Promoter Regions, Genetic/physiology

KW - Salmonella typhimurium/genetics

KW - Transcription Factors/genetics

KW - Transcription, Genetic

UR - https://journals.asm.org/doi/10.1128/JB.00218-10

U2 - 10.1128/JB.00218-10

DO - 10.1128/JB.00218-10

M3 - Article

C2 - 20472787

SN - 0021-9193

VL - 192

SP - 3824

EP - 3828

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 14

ER -

Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site

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Keywords

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