Endofluorescence Imaging of Murine Hepatocellular Carcinoma Cell Culture by Fluorescence Lifetime Microscopy with Modulated CMOS Camera

Elena Potapova; Evgeny Zherebtsov; Ksenia Kandurova; Alexander Palalov; Viktor Dremin; Andrey Dunaev

doi:10.18287/JBPE22.08.010303

Endofluorescence Imaging of Murine Hepatocellular Carcinoma Cell Culture by Fluorescence Lifetime Microscopy with Modulated CMOS Camera

Elena Potapova^*, Evgeny Zherebtsov, Ksenia Kandurova, Alexander Palalov, Viktor Dremin, Andrey Dunaev

^*Corresponding author for this work

Aston Institute of Photonic Technologies (AiPT)

Research output: Contribution to journal › Article › peer-review

Abstract

In this work, we propose the results of pilot studies on using the modulated CMOS camera for the imaging of autofluorescence in murine hepatocellular carcinoma cells with excitation in UV light. Here, we assess the capabilities of the imaging system to detect changes in the NAD(P)H fractions produced and utilised in the glycolysis, pentose phosphate pathway and oxidative phosphorylation. Our results suggest that the camera and the system based on one possess a sufficient margin of SNR and sensitivity to detect the cellular metabolic changes associated with the metabolic pathways mentioned.

Original language	English
Article number	010303
Number of pages	7
Journal	Journal of Biomedical Photonics and Engineering
Volume	8
Issue number	1
DOIs	https://doi.org/10.18287/JBPE22.08.010303
Publication status	Published - 22 Mar 2022

Bibliographical note

Copyright The Authors 2022. This work is licensed under a Creative Commons Attribution 4.0 International License
Funding Information:
The authors acknowledge the support of the Russian Science Foundation under project No. 21-15-00325. The authors appreciate the technical support and valuable discussions of Dr. Gerhard Holst (PCO AG, Kelheim, Germany).

Keywords

frequency domain fluorescence lifetime imaging
hepatocellular carcinoma
metabolism

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10.18287/JBPE22.08.010303Licence: CC BY 4.0

Potapovaetal_2022_VoR
Copyright The Authors 2022. This work is licensed under a Creative Commons Attribution 4.0 International License
Final published version, 2.23 MBLicence: CC BY 4.0

Cite this

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title = "Endofluorescence Imaging of Murine Hepatocellular Carcinoma Cell Culture by Fluorescence Lifetime Microscopy with Modulated CMOS Camera",

abstract = "In this work, we propose the results of pilot studies on using the modulated CMOS camera for the imaging of autofluorescence in murine hepatocellular carcinoma cells with excitation in UV light. Here, we assess the capabilities of the imaging system to detect changes in the NAD(P)H fractions produced and utilised in the glycolysis, pentose phosphate pathway and oxidative phosphorylation. Our results suggest that the camera and the system based on one possess a sufficient margin of SNR and sensitivity to detect the cellular metabolic changes associated with the metabolic pathways mentioned.",

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AU - Potapova, Elena

AU - Zherebtsov, Evgeny

AU - Kandurova, Ksenia

AU - Palalov, Alexander

AU - Dremin, Viktor

AU - Dunaev, Andrey

N1 - Copyright The Authors 2022. This work is licensed under a Creative Commons Attribution 4.0 International License Funding Information: The authors acknowledge the support of the Russian Science Foundation under project No. 21-15-00325. The authors appreciate the technical support and valuable discussions of Dr. Gerhard Holst (PCO AG, Kelheim, Germany).

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Y1 - 2022/3/22

N2 - In this work, we propose the results of pilot studies on using the modulated CMOS camera for the imaging of autofluorescence in murine hepatocellular carcinoma cells with excitation in UV light. Here, we assess the capabilities of the imaging system to detect changes in the NAD(P)H fractions produced and utilised in the glycolysis, pentose phosphate pathway and oxidative phosphorylation. Our results suggest that the camera and the system based on one possess a sufficient margin of SNR and sensitivity to detect the cellular metabolic changes associated with the metabolic pathways mentioned.

AB - In this work, we propose the results of pilot studies on using the modulated CMOS camera for the imaging of autofluorescence in murine hepatocellular carcinoma cells with excitation in UV light. Here, we assess the capabilities of the imaging system to detect changes in the NAD(P)H fractions produced and utilised in the glycolysis, pentose phosphate pathway and oxidative phosphorylation. Our results suggest that the camera and the system based on one possess a sufficient margin of SNR and sensitivity to detect the cellular metabolic changes associated with the metabolic pathways mentioned.

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KW - metabolism

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Endofluorescence Imaging of Murine Hepatocellular Carcinoma Cell Culture by Fluorescence Lifetime Microscopy with Modulated CMOS Camera

Abstract

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Keywords

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