Expression and purification of recombinant G protein-coupled receptors: A review

Daniel N. Wiseman; Abigail Otchere; Jaimin H. Patel; Romez Uddin; Naomi L. Pollock; Sarah J. Routledge; Alice J. Rothnie; Cathy Slack; David R. Poyner; Roslyn M. Bill; Alan D. Goddard

doi:10.1016/j.pep.2019.105524

Expression and purification of recombinant G protein-coupled receptors: A review

Daniel N. Wiseman, Abigail Otchere, Jaimin H. Patel, Romez Uddin, Naomi L. Pollock, Sarah J. Routledge, Alice J. Rothnie, Cathy Slack, David R. Poyner, Roslyn M. Bill, Alan D. Goddard

Research output: Contribution to journal › Article › peer-review

Abstract

Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques.

Original language	English
Article number	105524
Journal	Protein Expression and Purification
Volume	167
Early online date	31 Oct 2019
DOIs	https://doi.org/10.1016/j.pep.2019.105524
Publication status	Published - 1 Mar 2020

Bibliographical note

Funding: We are grateful for support from BBSRC (BB/N007417/1, BB/P025927/1, BB/P022685/1, BB/M016668/1, BB/L015846/1, BB/M007529/1, BB/R02152X/1, BB/S004696/1 and BB/T001488/1), H2020 ERA CoBioTech (under grant agreement No. 722361) and H2020 MSCA COFUND (under grant agreement No. 847419).

This project has received funding from the European Union’s Horizon 2020 research and innovation programme under Marie Sklodowska-Curie grant agreement No 847419

© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).

Keywords

Expression
GPCR
Purification
Review
SMALP

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Expression and purification of recombinant G protein-coupled receptors: A review
© 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 2.48 MBLicence: CC BY-NC-ND 3.0
1-s2.0-S1046592819304930-main
© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/BY/4.0/).
Final published version, 1.04 MBLicence: CC BY 3.0

Cite this

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abstract = "Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient quantity and quality of protein for downstream analysis. As such, this review highlights the systems available for recombinant GPCR expression, with consideration of their advantages and disadvantages, as well as examples of receptors successfully expressed in these systems. Additionally, an overview is given on the use of detergents and the styrene maleic acid (SMA) co-polymer for membrane solubilisation, as well as purification techniques.",

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Expression and purification of recombinant G protein-coupled receptors: A review

Abstract

Bibliographical note

Keywords

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