Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Evgeny A. Zherebtsov; Viktor V. Dremin; Elena V. Zharkikh; Angelina Zherebtsova; Ilya Rafailov; Andrey V. Dunaev; Edik U. Rafailov; Natalia Bazieva; Valery V. Tuchin; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang

doi:10.1117/12.2290431

Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Evgeny A. Zherebtsov, Viktor V. Dremin, Elena V. Zharkikh, Angelina Zherebtsova, Ilya Rafailov, Andrey V. Dunaev, Edik U. Rafailov, Natalia Bazieva, Valery V. Tuchin, Kirill V. Larin (Editor), Martin J. Leahy (Editor), Ruikang K. Wang (Editor)

Research output: Unpublished contribution to conference › Other › peer-review

Abstract

To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.

Original language	English
Pages	58
DOIs	https://doi.org/10.1117/12.2290431
Publication status	Published - 26 Feb 2018
Event	Dynamics and Fluctuations in Biomedical Photonics XV - San Francisco, United States Duration: 27 Jan 2018 → 1 Feb 2018

Conference

Conference	Dynamics and Fluctuations in Biomedical Photonics XV
Period	27/01/18 → 1/02/18

Bibliographical note

Copyright 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Funding: European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 703145. Academyof Finland (grant 296408), RFBR according to the research project №18-02-00669 and grant of the President of the RussianFederation for state support of young Russian scientists № MK-3400.2018.8

Access to Document

10.1117/12.2290431

Fibre-optic probe for fluorescence
Copyright 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Final published version, 636 KB

Cite this

Zherebtsov, E. A., Dremin, V. V., Zharkikh, E. V., Zherebtsova, A., Rafailov, I., Dunaev, A. V., Rafailov, E. U., Bazieva, N., Tuchin, V. V., Larin, K. V. (Ed.), Leahy, M. J. (Ed.), & Wang, R. K. (Ed.) (2018). Fibre-optic probe for fluorescence diagnostics with blood influence compensation. 58. Dynamics and Fluctuations in Biomedical Photonics XV. https://doi.org/10.1117/12.2290431

@conference{75f3176804694287a3f82c395c839a89,

title = "Fibre-optic probe for fluorescence diagnostics with blood influence compensation",

abstract = "To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.",

author = "Zherebtsov, {Evgeny A.} and Dremin, {Viktor V.} and Zharkikh, {Elena V.} and Angelina Zherebtsova and Ilya Rafailov and Dunaev, {Andrey V.} and Rafailov, {Edik U.} and Natalia Bazieva and Tuchin, {Valery V.} and Larin, {Kirill V.} and Leahy, {Martin J.} and Wang, {Ruikang K.}",

note = "Copyright 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Funding: European Union's Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant agreement No. 703145. Academyof Finland (grant 296408), RFBR according to the research project №18-02-00669 and grant of the President of the RussianFederation for state support of young Russian scientists № MK-3400.2018.8; Dynamics and Fluctuations in Biomedical Photonics XV ; Conference date: 27-01-2018 Through 01-02-2018",

year = "2018",

month = feb,

day = "26",

doi = "10.1117/12.2290431",

language = "English",

pages = "58",

}

Zherebtsov, EA, Dremin, VV, Zharkikh, EV, Zherebtsova, A, Rafailov, I, Dunaev, AV, Rafailov, EU, Bazieva, N, Tuchin, VV, Larin, KV (ed.), Leahy, MJ (ed.) & Wang, RK (ed.) 2018, 'Fibre-optic probe for fluorescence diagnostics with blood influence compensation', Dynamics and Fluctuations in Biomedical Photonics XV, 27/01/18 - 1/02/18 pp. 58. https://doi.org/10.1117/12.2290431

TY - CONF

T1 - Fibre-optic probe for fluorescence diagnostics with blood influence compensation

AU - Zherebtsov, Evgeny A.

AU - Dremin, Viktor V.

AU - Zharkikh, Elena V.

AU - Zherebtsova, Angelina

AU - Rafailov, Ilya

AU - Dunaev, Andrey V.

AU - Rafailov, Edik U.

AU - Bazieva, Natalia

AU - Tuchin, Valery V.

A2 - Larin, Kirill V.

A2 - Leahy, Martin J.

A2 - Wang, Ruikang K.

N1 - Copyright 2018 Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Funding: European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 703145. Academyof Finland (grant 296408), RFBR according to the research project №18-02-00669 and grant of the President of the RussianFederation for state support of young Russian scientists № MK-3400.2018.8

PY - 2018/2/26

Y1 - 2018/2/26

N2 - To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.

AB - To minimise the influence of blood content on the fluorescence measurements in vivo, a fibre optical probe combining fluorescence and diffuse reflectance measurements was developed. For the inverse solution of the blood content recovery, a set of neural networks trained by the Monte Carlo generated learning set was used. An approach of fluorescence measurements triggered by simultaneous real-time measurements of blood content in living tissue during moderate changes in contact pressure of the optic probe is proposed. The method allows one to decrease the necessary pressure on the probe as well as increase the repeatability of the measurements. The developed approach was verified in a series of experiments on volunteers with fluorescence excitation at 365 nm and 450 nm. The proposed technology is of particular interest in the development of new fluorescence-based optical biopsy systems.

UR - https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10493/2290431/Fibre-optic-probe-for-fluorescence-diagnostics-with-blood-influence-compensation/10.1117/12.2290431.full

U2 - 10.1117/12.2290431

DO - 10.1117/12.2290431

M3 - Other

SP - 58

T2 - Dynamics and Fluctuations in Biomedical Photonics XV

Y2 - 27 January 2018 through 1 February 2018

ER -

Fibre-optic probe for fluorescence diagnostics with blood influence compensation

Abstract

Conference

Bibliographical note

Access to Document

Other files and links

Fingerprint

Cite this