Force generation and protease gene expression in organotypic co-cultures of fibroblasts and keratinocytes

Ivan B Wall, Navneet Bhadal, Simon Broad, Simon A Whawell, Vivek Mudera, Mark P Lewis

Research output: Contribution to journalArticlepeer-review


Fibroblast-epithelium interactions are crucial for successful tissue engineering of skin and oral mucosal equivalents. In this study, we assessed early force generation in organotypic fibroblast-epithelium co-cultures, using normal human keratinocytes (NHK) and HPV16-transformed (UP) cells. During the initial 2 h period, organotypic co-cultures containing both epithelial cell types produced significantly more force than fibroblasts alone (p < 0.05). After 2 h, the epithelial contribution became diminished and did not significantly contribute to intrinsic force generation by fibroblasts, and no differences were observed when using UP vs. NHK. We then measured protease gene expression at the end of the experimental period. Distinct differences were evident in protease expression both between NHK-human skin fibroblast (HSF) vs. UP-HSF co-cultures and compared to fibroblasts alone. We conclude that whilst the very early contractile response of fibroblasts is enhanced by the overlying epithelium, this becomes diminished as the fibroblast response becomes predominant and it does contribute to tissue remodelling via regulation of protease expression.

Original languageEnglish
Pages (from-to)647-650
Number of pages4
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number8
Publication statusPublished - Dec 2009


  • Cells, Cultured
  • Coculture Techniques/methods
  • Fibroblasts/cytology
  • Gene Expression Regulation
  • Humans
  • Keratinocytes/cytology
  • Matrix Metalloproteinase 2/metabolism
  • Matrix Metalloproteinase 9/metabolism
  • Peptide Hydrolases/biosynthesis
  • Receptors, Urokinase Plasminogen Activator/metabolism
  • Skin/metabolism
  • Stress, Mechanical
  • Time Factors
  • Tissue Engineering/methods
  • Urokinase-Type Plasminogen Activator/metabolism


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