H2S regulation of nitric oxide metabolism

Gopi K. Kolluru, Shuai Yuan, Xinggui Shen, Christopher G. Kevil

Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review


Nitric oxide (NO) and hydrogen sulfide (H2S) are two major gaseous signaling molecules that regulate diverse physiological functions. Recent publications indicate the regulatory role of H2S on NO metabolism. In this chapter, we discuss the latest findings on H2S-NO interactions through formation of novel chemical derivatives and experimental approaches to study these adducts. This chapter also addresses potential H2S interference on various NO detection techniques, along with precautions for analyzing biological samples from various sources. This information will facilitate critical evaluation and clearer insight into H2S regulation of NO signaling and its influence on various physiological functions.

Original languageEnglish
Title of host publicationHydrogen sulfide in redox biology, part A
EditorsEnrique Cadenas, Lester Packer
Place of PublicationLondon (UK)
PublisherAcademic Press
Number of pages27
ISBN (Print)978-0-12-801512-4
Publication statusPublished - 9 Sept 2015

Publication series

NameMethods in Enzymology
PublisherAcademics Press
ISSN (Print)0076-6879


  • Western blotting
  • cultured cells
  • hydrogen sulfide
  • fluorescent dyes
  • gene expression
  • human umbilical vein endothelial cells
  • luminescent measurements
  • signal transduction
  • reference standards
  • nitric oxide
  • nitric oxide synthase
  • real-time polymerase chain reaction


Dive into the research topics of 'H2S regulation of nitric oxide metabolism'. Together they form a unique fingerprint.
  • Measurement of H2S in vivo and in vitro by the monobromobimane method

    Shen, X., Kolluru, G. K., Yuan, S. & Kevil, C. G., 2015, Hydrogen sulfide in redox biology, part A. Cadenas, E. & Packer, L. (eds.). London (UK): Academic Press, p. 31-45 15 p. (Methods in Enzymology; vol. 554).

    Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

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