TY - JOUR
T1 - Human heart failure biomarker immunosensor based on excessively tilted fiber gratings
AU - Luo, Binbin
AU - Wu, Shengxi
AU - Zhang, Zhonghao
AU - Zou, Wengen
AU - Shi, Shenghui
AU - Zhao, Mingfu
AU - Zhong, Nianbing
AU - Liu, Yong
AU - Zou, Xue
AU - Wang, Lingling
AU - Chai, Weina
AU - Hu, Chuanmin
AU - Zhang, Lin
N1 - © 2016 Optical Society of America
PY - 2017/1/1
Y1 - 2017/1/1
N2 - A label-free immunosensor platform based on excessively tilted fiber gratings (Ex- TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NTproBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NTproBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
AB - A label-free immunosensor platform based on excessively tilted fiber gratings (Ex- TFGs) was developed for highly specific and fast detection of human N-terminal pro-B-type natriuretic peptide (NT-proBNP), which is considered a powerful biomarker for prognosis and risk stratification of heart failure (HF). High-purity anti-NT-proBNP monoclonal antibodies (MAbs) prepared in our laboratory were immobilized on fiber surface through the staphylococcal protein A (SPA) method for subsequent specific binding of the targeted NTproBNP. Utilizing fiber optic grating demodulation system (FOGDS), immunoassays were carried out in vitro by monitoring the resonance wavelength shift of Ex-TFG biosensor with immobilized anti-NT-proBNP MAbs. Lowest detectable concentration of ~0.5ng/mL for NTproBNP was obtained, and average sensitivity for NT-proBNP at a concentration range of 0~1.0 ng/mL was approximately 45.967 pm/(ng/mL). Several human serum samples were assessed by the proposed Ex-TFG biomarker sensor, with high specificity for NT-proBNP, indicating potential application in early diagnosing patients with acute HF symptoms.
KW - biological sensing and sensors
KW - fiber optics sensors
KW - medical optics and biotechnology
UR - https://www.osapublishing.org/boe/abstract.cfm?uri=boe-8-1-57
UR - http://www.scopus.com/inward/record.url?scp=85008932690&partnerID=8YFLogxK
U2 - 10.1364/BOE.8.000057
DO - 10.1364/BOE.8.000057
M3 - Article
AN - SCOPUS:85008932690
SN - 2156-7085
VL - 8
SP - 57
EP - 67
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 1
ER -