In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas

P. Olszewski; T. C. Willett; E. Theodosiou; O. R T Thomas; J. L. Walsh

doi:10.1063/1.4807391

In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas

P. Olszewski, T. C. Willett, E. Theodosiou, O. R T Thomas^*, J. L. Walsh

^*Corresponding author for this work

College of Engineering and Physical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O₂ plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.

Original language	English
Article number	204104
Journal	Applied Physics Letters
Volume	102
Issue number	20
Early online date	20 May 2013
DOIs	https://doi.org/10.1063/1.4807391
Publication status	Published - 20 May 2013

Bibliographical note

© 2013 AIP Publishing LLC.

Funding: Biotechnology and Biological Sciences Research Council/Bioprocessing Research Industry Club (BRIC) programme (Grant No. BB/F004982/1), and the Engineering and Physical Sciences Research Council (Grant No. EP/J005894/1).

Keywords

Chromatography Plasma materials processing Proteins Atmospheric pressure plasma Atmospheric temperature

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title = "In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas",

abstract = "Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.",

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AU - Olszewski, P.

AU - Willett, T. C.

AU - Theodosiou, E.

AU - Thomas, O. R T

AU - Walsh, J. L.

N1 - © 2013 AIP Publishing LLC. Funding: Biotechnology and Biological Sciences Research Council/Bioprocessing Research Industry Club (BRIC) programme (Grant No. BB/F004982/1), and the Engineering and Physical Sciences Research Council (Grant No. EP/J005894/1).

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AB - Efficient manufacturing of increasingly sophisticated biopharmaceuticals requires the development of new breeds of chromatographic materials featuring two or more layers, with each layer affording different functions. This letter reports the in situ modification of a commercial beaded anion exchange adsorbent using atmospheric pressure plasma generated within gas bubbles. The results show that exposure to He-O2 plasma in this way yields significant reductions in the surface binding of plasmid DNA to the adsorbent exterior, with minimal loss of core protein binding capacity; thus, a bi-layered chromatography material exhibiting both size excluding and anion exchange functionalities within the same bead is produced.

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In situ modification of chromatography adsorbents using cold atmospheric pressure plasmas

Abstract

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