Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro

Lívia Santos; L. S. Gonçalves; Shirin Bagheri-Hanei; Gabriella Berwig Möller; Craig Sale; Ruth M. James; Guilherme Giannini Artioli

doi:10.1007/s00726-021-03090-9

Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro

Lívia Santos, L. S. Gonçalves, Shirin Bagheri-Hanei, Gabriella Berwig Möller, Craig Sale, Ruth M. James, Guilherme Giannini Artioli^*

^*Corresponding author for this work

Chemical Engineering & Applied Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L⁻¹), with insulin (100 µU·mL⁻¹) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L⁻¹) but not higher (700 µmol·L⁻¹) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin.

Original language	English
Pages (from-to)	1763-1766
Number of pages	4
Journal	Amino Acids
Volume	53
Issue number	11
Early online date	21 Oct 2021
DOIs	https://doi.org/10.1007/s00726-021-03090-9
Publication status	Published - Nov 2021

Bibliographical note

Funding Information:
L.S.G: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); G.G.A: São Paulo Research Foundation—FAPESP (#2014/11948-8; 2019/25032-9). This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES-Finance Code 001).

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Keywords

Carnosine
Insulin
Taurine transporter
β-alanine

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10.1007/s00726-021-03090-9Licence: CC BY 3.0

Santos2021_Article_InsulinStimulatesΒ-alanineUpta
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Final published version, 714 KBLicence: CC BY 3.0

Cite this

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abstract = "We evaluated whether insulin could stimulate β-alanine uptake by skeletal muscle cells in vitro. Mouse myoblasts (C2C12) (n = 3 wells per condition) were cultured with β-alanine (350 or 700 µmol·L−1), with insulin (100 µU·mL−1) either added to the media or not. Insulin stimulated the β-alanine uptake at the lower (350 µmol·L−1) but not higher (700 µmol·L−1) β-alanine concentration in culture medium, indicating that transporter saturation might blunt the stimulatory effects of insulin.",

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Insulin stimulates β-alanine uptake in skeletal muscle cells in vitro

Abstract

Bibliographical note

Keywords

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