Low-cost fabrication of PPIX liquid phantoms for use in fluorescence Measurements

Valery Shupletsov; Margarita Mikenkina; Evgeny Zherebtsov; Viktor Dremin; Alexander Bykov; Elena Potapova; Andrey Dunaev; Igor Meglinski

doi:10.1117/12.2626389

Low-cost fabrication of PPIX liquid phantoms for use in fluorescence Measurements

Valery Shupletsov^*, Margarita Mikenkina, Evgeny Zherebtsov, Viktor Dremin, Alexander Bykov, Elena Potapova, Andrey Dunaev, Igor Meglinski

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

Abstract

This article presents a liquid phantom technology that simulates the fluorescent properties of protoporphyrin IX (PPIX). A technology was developed for the isolation of PPIX from dark egg shells with a final concentration close to the real values in human tissues. A comparative analysis of the transmittance coefficient of the manufactured phantom as well as the fluorescence spectra measured through the combined use of a CCD spectrometer and a hyperspectral camera is presented as results.

Original language	English
Article number	121920U
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12192
DOIs	https://doi.org/10.1117/12.2626389
Publication status	Published - 29 Apr 2022
Event	Saratov Fall Meeting 2021: Optical Technologies for Biology and Medicine - Saratov, Russian Federation Duration: 27 Sept 2021 → 1 Oct 2021

Bibliographical note

Copyright 2022 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Funding Information:
The authors acknowledge the support of the Russian Science Foundation under project No. 21-15-00325.

Keywords

fluorescence imaging
fluorescence spectroscopy
optical phantom
protoporphyrin IX

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10.1117/12.2626389

Low-cost fabrication of PPIX liquid phantoms
Copyright 2022 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
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Cite this

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abstract = "This article presents a liquid phantom technology that simulates the fluorescent properties of protoporphyrin IX (PPIX). A technology was developed for the isolation of PPIX from dark egg shells with a final concentration close to the real values in human tissues. A comparative analysis of the transmittance coefficient of the manufactured phantom as well as the fluorescence spectra measured through the combined use of a CCD spectrometer and a hyperspectral camera is presented as results.",

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AU - Shupletsov, Valery

AU - Mikenkina, Margarita

AU - Zherebtsov, Evgeny

AU - Dremin, Viktor

AU - Bykov, Alexander

AU - Potapova, Elena

AU - Dunaev, Andrey

AU - Meglinski, Igor

N1 - Copyright 2022 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Funding Information: The authors acknowledge the support of the Russian Science Foundation under project No. 21-15-00325.

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N2 - This article presents a liquid phantom technology that simulates the fluorescent properties of protoporphyrin IX (PPIX). A technology was developed for the isolation of PPIX from dark egg shells with a final concentration close to the real values in human tissues. A comparative analysis of the transmittance coefficient of the manufactured phantom as well as the fluorescence spectra measured through the combined use of a CCD spectrometer and a hyperspectral camera is presented as results.

AB - This article presents a liquid phantom technology that simulates the fluorescent properties of protoporphyrin IX (PPIX). A technology was developed for the isolation of PPIX from dark egg shells with a final concentration close to the real values in human tissues. A comparative analysis of the transmittance coefficient of the manufactured phantom as well as the fluorescence spectra measured through the combined use of a CCD spectrometer and a hyperspectral camera is presented as results.

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Low-cost fabrication of PPIX liquid phantoms for use in fluorescence Measurements

Abstract

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Keywords

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