Low-cost fabrication of PPIX liquid phantoms for use in fluorescence Measurements

Valery Shupletsov*, Margarita Mikenkina, Evgeny Zherebtsov, Viktor Dremin, Alexander Bykov, Elena Potapova, Andrey Dunaev, Igor Meglinski

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review


This article presents a liquid phantom technology that simulates the fluorescent properties of protoporphyrin IX (PPIX). A technology was developed for the isolation of PPIX from dark egg shells with a final concentration close to the real values in human tissues. A comparative analysis of the transmittance coefficient of the manufactured phantom as well as the fluorescence spectra measured through the combined use of a CCD spectrometer and a hyperspectral camera is presented as results.

Original languageEnglish
Article number121920U
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Publication statusPublished - 29 Apr 2022
EventSaratov Fall Meeting 2021: Optical Technologies for Biology and Medicine - Saratov, Russian Federation
Duration: 27 Sept 20211 Oct 2021

Bibliographical note

Copyright 2022 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Funding Information:
The authors acknowledge the support of the Russian Science Foundation under project No. 21-15-00325.


  • fluorescence imaging
  • fluorescence spectroscopy
  • optical phantom
  • protoporphyrin IX


Dive into the research topics of 'Low-cost fabrication of PPIX liquid phantoms for use in fluorescence Measurements'. Together they form a unique fingerprint.

Cite this