Lymphocyte peroxiredoxin-2 levels are depleted one week after ultra-endurance exercise

J.E. Turner, S.J. Bennett, J.A. Bosch, S. Aldred, H.R. Griffiths

Research output: Contribution to journalConference abstractpeer-review


Peroxiredoxin-2 (PRDX-2) belongs to a family of thiol containing proteins and is important for antioxidant defense, redox signaling and cell function. This study examined whether lymphocyte PRDX-2 levels are altered over one month following ultra-endurance exercise. Nine middle-aged men participated in a 145 mile ultra-endurance running race event. Blood drawing was undertaken immediately before, upon completion/retirement, and at one, seven and twenty eight-days following the race. PRDX-2 levels were examined at each time-point, for all participants (n=9) by reducing SDS-PAGE and western blotting. Further analysis using non-reducing SDS-PAGE and western blotting was undertaken in a sub-group of men who completed the race (n = 4) to investigate PRDX-2 oligomeric state (indicative of oxidation state). Ultra-endurance exercise caused a significant alteration in lymphocyte PRDX-2 levels (F(4,32) 3.409, p=0.020, η2 =0.299): seven-days after the race PRDX-2 levels fell by 70% (p=0.013) and at twenty eight-days after the race returned to near-normal levels. PRDX-2 dimers (intracellular reduced PRDX-2 monomers) in three of the four participants, who finished the race, were increased upon race completion. Furthermore, PRDX-2 monomers (intracellular over-oxidized PRDX-2 monomers) in two of these four participants were present upon race completion, but absent seven-days after the race. This study found that PRDX-2 levels in lymphocytes were reduced below normal levels seven-days after an ultra-endurance running race. We suggest that excessive reactive oxygen species production, induced by ultra-endurance exercise may, in part, explain the depletion of lymphocyte PRDX-2 by triggering its turnover after oxidation.
Original languageEnglish
Article numberPP90
Pages (from-to)S56
Number of pages1
JournalFree Radical Biology and Medicine
Issue numberSuppl.1
Publication statusPublished - 20 Sept 2013
EventSFRR - Europe 2013 meeting - Athens, Greece
Duration: 23 Sept 201325 Sept 2013

Bibliographical note

SFRR - Europe 2013 Meeting "The new era of -omics in Free Radicals in Biology and Medicine", 23 - 25 Sep 2013, Athens, Greece.


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