Measurement of H2S in vivo and in vitro by the monobromobimane method

Xinggui Shen; Gopi K Kolluru; Shuai Yuan; Christopher G. Kevil

doi:10.1016/bs.mie.2014.11.039

Measurement of H₂S in vivo and in vitro by the monobromobimane method

Xinggui Shen, Gopi K Kolluru, Shuai Yuan, Christopher G. Kevil

Aston Medical School

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Abstract

The gasotransmitter hydrogen sulfide (H2S) is known as an important regulator in several physiological and pathological responses. Among the challenges facing the field is the accurate and reliable measurement of hydrogen sulfide bioavailability. We have reported an approach to discretely measure sulfide and sulfide pools using the monobromobimane (MBB) method coupled with reversed phase high-performance liquid chromatography (RP-HPLC). The method involves the derivatization of sulfide with excess MBB under precise reaction conditions at room temperature to form sulfide dibimane (SDB). The resultant fluorescent SDB is analyzed by RP-HPLC using fluorescence detection with the limit of detection for SDB (2 nM). Care must be taken to avoid conditions that may confound H2S measurement with this method. Overall, RP-HPLC with fluorescence detection of SDB is a useful and powerful tool to measure biological sulfide levels.

Original language	English
Title of host publication	Hydrogen sulfide in redox biology, part A
Editors	Enrique Cadenas, Lester Packer
Place of Publication	London (UK)
Publisher	Academic Press
Pages	31-45
Number of pages	15
ISBN (Print)	978-0-12-801512-4
DOIs	https://doi.org/10.1016/bs.mie.2014.11.039
Publication status	Published - 2015

Publication series

Name	Methods in Enzymology
Publisher	Academics Press
Volume	554
ISSN (Print)	0076-6879

Keywords

high pressure liquid chromatography
bicyclo compounds
fluorescence spectrometry
fluorescent dyes
electrospray ionization
hydrogen sulfide
reference standards
spectrometry
mass

Access to Document

10.1016/bs.mie.2014.11.039

1 Chapter (peer-reviewed)

H₂S regulation of nitric oxide metabolism
Kolluru, G. K., Yuan, S., Shen, X. & Kevil, C. G., 9 Sept 2015, Hydrogen sulfide in redox biology, part A. Cadenas, E. & Packer, L. (eds.). London (UK): Academic Press, p. 271-297 27 p. (Methods in Enzymology; vol. 554).
Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

Cite this

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title = "Measurement of H2S in vivo and in vitro by the monobromobimane method",

abstract = "The gasotransmitter hydrogen sulfide (H2S) is known as an important regulator in several physiological and pathological responses. Among the challenges facing the field is the accurate and reliable measurement of hydrogen sulfide bioavailability. We have reported an approach to discretely measure sulfide and sulfide pools using the monobromobimane (MBB) method coupled with reversed phase high-performance liquid chromatography (RP-HPLC). The method involves the derivatization of sulfide with excess MBB under precise reaction conditions at room temperature to form sulfide dibimane (SDB). The resultant fluorescent SDB is analyzed by RP-HPLC using fluorescence detection with the limit of detection for SDB (2 nM). Care must be taken to avoid conditions that may confound H2S measurement with this method. Overall, RP-HPLC with fluorescence detection of SDB is a useful and powerful tool to measure biological sulfide levels.",

keywords = "high pressure liquid chromatography, bicyclo compounds, fluorescence spectrometry, fluorescent dyes, electrospray ionization, hydrogen sulfide, reference standards, spectrometry, mass",

author = "Xinggui Shen and Kolluru, {Gopi K} and Shuai Yuan and Kevil, {Christopher G.}",

year = "2015",

doi = "10.1016/bs.mie.2014.11.039",

language = "English",

isbn = "978-0-12-801512-4",

series = "Methods in Enzymology",

publisher = "Academic Press",

pages = "31--45",

editor = "Enrique Cadenas and Lester Packer",

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Measurement of H₂S in vivo and in vitro by the monobromobimane method. / Shen, Xinggui; Kolluru, Gopi K; Yuan, Shuai et al.
Hydrogen sulfide in redox biology, part A. ed. / Enrique Cadenas; Lester Packer. London (UK): Academic Press, 2015. p. 31-45 (Methods in Enzymology; Vol. 554).

Research output: Chapter in Book/Published conference output › Chapter (peer-reviewed) › peer-review

TY - CHAP

T1 - Measurement of H2S in vivo and in vitro by the monobromobimane method

AU - Shen, Xinggui

AU - Kolluru, Gopi K

AU - Yuan, Shuai

AU - Kevil, Christopher G.

PY - 2015

Y1 - 2015

N2 - The gasotransmitter hydrogen sulfide (H2S) is known as an important regulator in several physiological and pathological responses. Among the challenges facing the field is the accurate and reliable measurement of hydrogen sulfide bioavailability. We have reported an approach to discretely measure sulfide and sulfide pools using the monobromobimane (MBB) method coupled with reversed phase high-performance liquid chromatography (RP-HPLC). The method involves the derivatization of sulfide with excess MBB under precise reaction conditions at room temperature to form sulfide dibimane (SDB). The resultant fluorescent SDB is analyzed by RP-HPLC using fluorescence detection with the limit of detection for SDB (2 nM). Care must be taken to avoid conditions that may confound H2S measurement with this method. Overall, RP-HPLC with fluorescence detection of SDB is a useful and powerful tool to measure biological sulfide levels.

AB - The gasotransmitter hydrogen sulfide (H2S) is known as an important regulator in several physiological and pathological responses. Among the challenges facing the field is the accurate and reliable measurement of hydrogen sulfide bioavailability. We have reported an approach to discretely measure sulfide and sulfide pools using the monobromobimane (MBB) method coupled with reversed phase high-performance liquid chromatography (RP-HPLC). The method involves the derivatization of sulfide with excess MBB under precise reaction conditions at room temperature to form sulfide dibimane (SDB). The resultant fluorescent SDB is analyzed by RP-HPLC using fluorescence detection with the limit of detection for SDB (2 nM). Care must be taken to avoid conditions that may confound H2S measurement with this method. Overall, RP-HPLC with fluorescence detection of SDB is a useful and powerful tool to measure biological sulfide levels.

KW - high pressure liquid chromatography

KW - bicyclo compounds

KW - fluorescence spectrometry

KW - fluorescent dyes

KW - electrospray ionization

KW - hydrogen sulfide

KW - reference standards

KW - spectrometry

KW - mass

U2 - 10.1016/bs.mie.2014.11.039

DO - 10.1016/bs.mie.2014.11.039

M3 - Chapter (peer-reviewed)

C2 - 25725514

SN - 978-0-12-801512-4

T3 - Methods in Enzymology

SP - 31

EP - 45

BT - Hydrogen sulfide in redox biology, part A

A2 - Cadenas, Enrique

A2 - Packer, Lester

PB - Academic Press

CY - London (UK)

ER -

Measurement of H2S in vivo and in vitro by the monobromobimane method

Abstract

Publication series

Keywords

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Research output

H2S regulation of nitric oxide metabolism

Cite this

Measurement of H₂S in vivo and in vitro by the monobromobimane method

H₂S regulation of nitric oxide metabolism