Abstract
The fabrication of artificial tissue and organ models is one of the important directions of the development of modern biomedicine. Assessment of the morphology, maturation, and viability is an important part of such developments. Here, we report on the validation of our custom-build fluorescence spectroscopy (FS) system with optical coherence tomography (OCT) for assessing the metabolism and morphology of the full-thickness skin equivalence (FSE) model. FS along with OCT has been used for the metabolic activity evaluation of the developed FSE model and 3D imaging of its structure. Thus, we have developed a multimodal optical system that can be used in the future for a full-profile assessment of the maturation and viability of 3D-printed models of biological tissues in time-course development.
| Original language | English |
|---|---|
| Article number | 126290T |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 12629 |
| DOIs | |
| Publication status | Published - 11 Aug 2023 |
| Event | Emerging Technologies for Cell and Tissue Characterization II 2023 - Munich, Germany Duration: 28 Jun 2023 → 29 Jun 2023 |
Bibliographical note
Funding Information:This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme under Grant Agreement No. 951890 (PLATFORMA project).
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Keywords
- artificial full-thickness skin model
- fibroblasts
- fluorescence spectroscopy
- keratinocytes
- optical coherence tomography
- scaffolds