Modular peptide binders – development of a predictive technology as alternative for reagent antibodies

Florian J. Gisdon, Josef P. Kynast, Merve Ayyildiz, Anna V. Hine, Andreas Plückthun, Birte Höcker*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


Current biomedical research and diagnostics critically depend on detection agents for specific recognition and quantification of protein molecules. Monoclonal antibodies have been used for this purpose over decades and facilitated numerous biological and biomedical investigations. Recently, however, it has become apparent that many commercial reagent antibodies lack specificity or do not recognize their target at all. Thus, synthetic alternatives are needed whose complex designs are facilitated by multidisciplinary approaches incorporating experimental protein engineering with computational modeling. Here, we review the status of such an engineering endeavor based on the modular armadillo repeat protein scaffold and discuss challenges in its implementation.

Original languageEnglish
Pages (from-to)535-543
JournalBiological Chemistry
Issue number5-6
Early online date28 Jan 2022
Publication statusPublished - 1 Apr 2022

Bibliographical note

© 2022 Florian J. Gisdon et al., published by De Gruyter. This work is licensed under the Creative Commons Attribution 4.0
International License.

Funding: This work was supported by H2020-FETopen-RIA grant agreement 764434 (‘PRe-ART’)


  • Clinical Biochemistry
  • Molecular Biology
  • Biochemistry


Dive into the research topics of 'Modular peptide binders – development of a predictive technology as alternative for reagent antibodies'. Together they form a unique fingerprint.

Cite this