TY - JOUR
T1 - Modulation of SERCA in the chronic phase of adjuvant arthritis as a possible adaptation mechanism of redox imbalance
AU - Strosova, Miriam
AU - Karlovska, Jana
AU - Spickett, Corinne M.
AU - Orszagova, Zuzana
AU - Ponist, Silvester
AU - Bauerova, Katarina
AU - Mihalova, Danica
AU - Horakova, Lubica
PY - 2009/9
Y1 - 2009/9
N2 - Adjuvant arthritis (AA) is a condition that involves systemic oxidative stress. Unexpectedly, it was found that sarcoplasmic reticulum Ca2 +-ATPase (SERCA) activity was elevated in muscles of rats with AA compared to controls, suggesting possible conformational changes in the enzyme. There was no alteration in the nucleotide binding site but rather in the transmembrane domain according to the tryptophan polar/non-polar fluorescence ratio. Higher relative expression of SERCA, higher content of nitrotyrosine but no increase in phospholipid oxidation in AA SR was found. In vitro treatments of SR with HOCl showed that in AA animals SERCA activity was more susceptible to oxidative stress, but SR phospholipids were more resistant and SERCA could also be activated by phosphatidic acid. It was concluded that increased SERCA activity in AA was due to increased levels of SERCA protein and structural changes to the protein, probably induced by direct and specific oxidation involving reactive nitrogen species.
AB - Adjuvant arthritis (AA) is a condition that involves systemic oxidative stress. Unexpectedly, it was found that sarcoplasmic reticulum Ca2 +-ATPase (SERCA) activity was elevated in muscles of rats with AA compared to controls, suggesting possible conformational changes in the enzyme. There was no alteration in the nucleotide binding site but rather in the transmembrane domain according to the tryptophan polar/non-polar fluorescence ratio. Higher relative expression of SERCA, higher content of nitrotyrosine but no increase in phospholipid oxidation in AA SR was found. In vitro treatments of SR with HOCl showed that in AA animals SERCA activity was more susceptible to oxidative stress, but SR phospholipids were more resistant and SERCA could also be activated by phosphatidic acid. It was concluded that increased SERCA activity in AA was due to increased levels of SERCA protein and structural changes to the protein, probably induced by direct and specific oxidation involving reactive nitrogen species.
KW - adjuvant arthritis
KW - oxidative stress
KW - Ca2 +-ATPase
KW - sarcoplasmic reticulum
KW - nitrotyrosine
KW - Pharmacy and materia medica
KW - Microbiology
UR - http://www.scopus.com/inward/record.url?scp=70349312661&partnerID=8YFLogxK
UR - http://informahealthcare.com/doi/abs/10.1080/10715760903089708
U2 - 10.1080/10715760903089708
DO - 10.1080/10715760903089708
M3 - Article
SN - 1071-5762
VL - 43
SP - 852
EP - 864
JO - Free Radical Research
JF - Free Radical Research
IS - 9
ER -