Monitoring of interaction of low-frequency electric field with biological tissues upon optical clearing with optical coherence tomography

Adrián F. Peña, Alexander Doronin, Valery V. Tuchin, Igor Meglinski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The influence of a low-frequency electric field applied to soft biological tissues ex vivo at normal conditions and upon the topical application of optical clearing agents has been studied by optical coherence tomography (OCT). The electro-kinetic response of tissues has been observed and quantitatively evaluated by the double correlation OCT approach, utilizing consistent application of an adaptive Wiener filtering and Fourier domain correlation algorithm. The results show that fluctuations, induced by the electric field within the biological tissues are exponentially increased in time. We demonstrate that in comparison to impedance measurements and the mapping of the temperature profile at the surface of the tissue samples, the double correlation OCT approach is much more sensitive to the changes associated with the tissues' electro-kinetic response. We also found that topical application of the optical clearing agent reduces the tissues' electro-kinetic response and is cooling the tissue, thus reducing the temperature induced by the electric current by a few degrees. We anticipate that dcOCT approach can find a new application in bioelectrical impedance analysis and monitoring of the electric properties of biological tissues, including the resistivity of high water content tissues and its variations.

Original languageEnglish
Article number086002
JournalJournal of Biomedical Optics
Volume19
Issue number8
DOIs
Publication statusPublished - 6 Aug 2014

Bibliographical note

Copyright 2014 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

Keywords

  • bioelectrical impedance.
  • biological tissues
  • electric field
  • electro-kinetic response
  • optical clearing
  • optical coherence tomography
  • temperature

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