Oxygen radical induced alterations in polyclonal IgG

Helen R. Griffiths, Joseph Lunec, C.A. Gee, R.L. Willson

Research output: Contribution to journalArticlepeer-review


In the sera and synovial fluid of patients with rheumatoid arthritis, part of the IgG fraction is found in an aggregated and fluorescent form. Oxygen-free radicals have been implicated in this denaturation, although the precise radical species responsible is unknown. In this work, oxygen-free radicals generated radiolytically were allowed to attack polyclonal IgG in solution. OH radicals induced aggregation of the monomer and a new fluorescence appeared in the visible region (Ex 360 nm, Em 454 nm). The superoxide radical anion was found to be inert in both these respects, whilst peroxy radicals induced autofluorescence without concomitant aggregation. The results suggest that OH.and/or peroxy radical attack may be an in vivo mechanism for IgG denaturation.
Original languageEnglish
Pages (from-to)155-158
Number of pages4
JournalFEBS Letters
Issue number1-2
Publication statusPublished - 28 Mar 1988


  • oxygen-free radical
  • fluorescence
  • IgG


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