Particulate material for purification of bio-macromolecules

Morten A. Olander (Inventor), Allan O.F. Lihme (Inventor), Timothy J. Hobley (Inventor), Marcos Simon (Inventor), Irene Theodossiou (Inventor), Owen R.T. Thomas (Inventor)

Research output: Patent


The present invention relates to particulate material having a density of at least 2.5 g/ml, where the particles of the particulate material have an average diameter of 5-75 mum, and the particles of the particulate material are essentially constructed of a polymeric base matrix, e.g. a polysaccharide such as agarose, and a non-porous core material, e.g. steel and titanium, said core material having a density of at least 3.0 g/ml, said polymeric base matrix including pendant groups which are positively charged at pH 4.0 or which are affinity ligands for a bio-molecule. Possible pendant groups include polyethyleneimine (PEI), diethylaminoethyl (DEAE) and quaternary aminoethyl (QAE). The materials are useful in expanded bed or fluidized bed chromatography processes, in particular for purification of bio-macromolecules such as plasmid DNA, chromosomal DNA, RNA, viral DNA, bacteria and viruses.
Original languageEnglish
Patent numberUS6783962 B1
Priority date26/03/99
Publication statusPublished - 31 Aug 2004


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