Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells

Gary Dever; Laura-Jayne Stewart; Andrew Pitt; Corinne M. Spickett

doi:10.1016/S0014-5793(03)00271-0

Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells

Gary Dever, Laura-Jayne Stewart, Andrew Pitt, Corinne M. Spickett

Research output: Contribution to journal › Article › peer-review

Abstract

Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (111,60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10100 muM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.

Original language	English
Pages (from-to)	245-250
Number of pages	6
Journal	FEBS Letters
Volume	540
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(03)00271-0
Publication status	Published - 10 Apr 2003

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Keywords

phosphatidylcholine
HOCl
oxidative stress
chlorohydrin
HL60
atherosclerosis
Pharmacy and materia medica

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10.1016/S0014-5793(03)00271-0

Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells
Articles published under an Elsevier user license are protected by copyright and may be used for non-commercial purposes. Users may access, download, copy, translate, text mine and data mine the articles provided that users: - Cite the article using an appropriate bibliographic citation and DOI - Use the article for non-commercial purposes - Maintain the integrity of the article - Retain copyright notices and links to these terms and conditions so it is clear to other users what can and cannot be done with the article - Ensure that, for any content in the article that is identified as belonging to a third party, any re-use complies with the copyright policies of that third party
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title = "Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells",

abstract = "Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (111,60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10100 muM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.",

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author = "Gary Dever and Laura-Jayne Stewart and Andrew Pitt and Spickett, {Corinne M.}",

note = "Articles published under an Elsevier user license are protected by copyright and may be used for non-commercial purposes. Users may access, download, copy, translate, text mine and data mine the articles provided that users: - Cite the article using an appropriate bibliographic citation (i.e. author(s), journal, article title, volume, issue, page numbers, DOI and the link to the definitive published version on ScienceDirect) - Use the article for non-commercial purposes - Maintain the integrity of the article - Retain copyright notices and links to these terms and conditions so it is clear to other users what can and cannot be done with the article - Ensure that, for any content in the article that is identified as belonging to a third party, any re-use complies with the copyright policies of that third party - Any translations, for which a prior translation agreement with Elsevier has not been established, must prominently display the statement: {"}This is an unofficial translation of an article that appeared in an Elsevier publication. Elsevier has not endorsed this translation.{"}",

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N2 - Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (111,60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10100 muM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.

AB - Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (111,60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10100 muM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.

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KW - HOCl

KW - oxidative stress

KW - chlorohydrin

KW - HL60

KW - atherosclerosis

KW - Pharmacy and materia medica

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DO - 10.1016/S0014-5793(03)00271-0

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SN - 0014-5793

VL - 540

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EP - 250

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells

Abstract

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Keywords

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