PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

Anupama Chembath; Mohammed Ashraf; Ben Wagstaffe; Yvonne  Stark; Erich Michel; Alina Rohrbach; Josef Kynast; Florian  Gisdon; Merve  Ayyildiz; Birte  Höcker; Andreas Plückthun; Anna V. Hine

PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

Anupama Chembath, Mohammed Ashraf, Ben Wagstaffe, Yvonne Stark, Erich Michel, Alina Rohrbach, Josef Kynast, Florian Gisdon, Merve Ayyildiz, Birte Höcker, Andreas Plückthun, Anna V. Hine^*

^*Corresponding author for this work

School of Biosciences

Research output: Unpublished contribution to conference › Poster › peer-review

Abstract

PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeat
proteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.

Original language	English
Publication status	Published - 4 May 2022
Event	PEGS Boston - The essential protein engineering and cell therapy summit - Boston, Boston, United States Duration: 2 May 2022 → 6 May 2022 https://www.pegsummit.com/

Conference

Conference	PEGS Boston - The essential protein engineering and cell therapy summit
Abbreviated title	PEGS Boston 2022
Country/Territory	United States
City	Boston
Period	2/05/22 → 6/05/22
Internet address	https://www.pegsummit.com/

Access to Document

https://www.pegsummit.com/poster-titles

1 Article

Modular binder technology by NGS-aided, high-resolution selection in yeast of designed armadillo modules
Stark, Y., Menard, F., Jeliazkov, J., Ernst, P., Chembath, A., Ashraf, M., Hine, A. V. & Plueckthun, A., 2 Jul 2024, In: Proceedings of the National Academy of Sciences of the United States of America. 121, 27, 11 p., e2318198121.
Research output: Contribution to journal › Article › peer-review

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Chembath, A., Ashraf, M., Wagstaffe, B., Stark, Y., Michel, E., Rohrbach, A., Kynast, J., Gisdon, F., Ayyildiz, M., Höcker, B., Plückthun, A., & Hine, A. V. (2022). PRe-ART - Creating Synthetic Replacements for Reagent Antibodies. Poster session presented at PEGS Boston - The essential protein engineering and cell therapy summit, Boston, Massachusetts, United States. https://www.pegsummit.com/poster-titles

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title = "PRe-ART - Creating Synthetic Replacements for Reagent Antibodies",

abstract = "PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeatproteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.",

author = "Anupama Chembath and Mohammed Ashraf and Ben Wagstaffe and Yvonne Stark and Erich Michel and Alina Rohrbach and Josef Kynast and Florian Gisdon and Merve Ayyildiz and Birte H{\"o}cker and Andreas Pl{\"u}ckthun and Hine, {Anna V.}",

year = "2022",

month = may,

day = "4",

language = "English",

note = " PEGS Boston - The essential protein engineering and cell therapy summit, PEGS Boston 2022 ; Conference date: 02-05-2022 Through 06-05-2022",

url = "https://www.pegsummit.com/",

}

Chembath, A , Ashraf, M, Wagstaffe, B, Stark, Y, Michel, E, Rohrbach, A, Kynast, J, Gisdon, F, Ayyildiz, M, Höcker, B, Plückthun, A & Hine, AV 2022, 'PRe-ART - Creating Synthetic Replacements for Reagent Antibodies', PEGS Boston - The essential protein engineering and cell therapy summit, Boston, United States, 2/05/22 - 6/05/22. <https://www.pegsummit.com/poster-titles>

TY - CONF

T1 - PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

AU - Chembath, Anupama

AU - Ashraf, Mohammed

AU - Wagstaffe, Ben

AU - Stark, Yvonne

AU - Michel, Erich

AU - Rohrbach, Alina

AU - Kynast, Josef

AU - Gisdon, Florian

AU - Ayyildiz, Merve

AU - Höcker, Birte

AU - Plückthun, Andreas

AU - Hine, Anna V.

PY - 2022/5/4

Y1 - 2022/5/4

N2 - PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeatproteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.

AB - PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeatproteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.

M3 - Poster

T2 - PEGS Boston - The essential protein engineering and cell therapy summit

Y2 - 2 May 2022 through 6 May 2022

ER -

PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

Abstract

Conference

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Research output

Modular binder technology by NGS-aided, high-resolution selection in yeast of designed armadillo modules

Cite this