PRe-ART - Creating Synthetic Replacements for Reagent Antibodies

Anupama Chembath, Mohammed Ashraf, Ben Wagstaffe, Yvonne Stark, Erich Michel, Alina Rohrbach, Josef Kynast, Florian Gisdon, Merve Ayyildiz, Birte Höcker, Andreas Plückthun, Anna V. Hine*

*Corresponding author for this work

Research output: Unpublished contribution to conferencePosterpeer-review


PRe-ART (Predictive Reagent Antibody Replacement Technology) aims to replace animal-derived, underperforming reagent antibodies with designed armadillo repeat
proteins (dArmRPs) for widespread use as protein-binding reagents, in biomedical research. These rationally designed, modular proteins bind linear target peptides in a conserved and extended conformation. Based on a designed and highly stable armadillo repeat protein, individual armadillo repeats are engineered by a combination of computational design, high-throughput mutagenesis and high throughput affinity screening to achieve single amino acid specificity. Variant repeats are then assembled according to the required target in a modular manner, ultimately to bind protein/peptide targets on demand.
Original languageEnglish
Publication statusPublished - 4 May 2022
Event PEGS Boston - The essential protein engineering and cell therapy summit - Boston, Boston, United States
Duration: 2 May 20226 May 2022


Conference PEGS Boston - The essential protein engineering and cell therapy summit
Abbreviated titlePEGS Boston 2022
Country/TerritoryUnited States
Internet address


Dive into the research topics of 'PRe-ART - Creating Synthetic Replacements for Reagent Antibodies'. Together they form a unique fingerprint.

Cite this