Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

Carmen Hierro-Iglesias, Cornelius O. Fatokun, Annie Chimphango, Richard Bayitse, Paula Helena Blanco Sanchez, Patricia Thornley, Alfred Fernandez-Castane*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.
Original languageEnglish
Article number111815
Number of pages13
JournalJournal of Environmental Chemical engineering
Volume12
Issue number1
Early online date25 Dec 2023
DOIs
Publication statusPublished - Feb 2024

Bibliographical note

This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

  • Acid hydrolysis
  • Cassava
  • Cupriavidus necator
  • Polyhydroxyalkanoates
  • Waste valorization

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