Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

Carmen Hierro-Iglesias; Cornelius O. Fatokun; Annie Chimphango; Richard Bayitse; Paula Helena Blanco Sanchez; Patricia Thornley; Alfred Fernandez-Castane

doi:10.1016/j.jece.2023.111815

Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

Carmen Hierro-Iglesias, Cornelius O. Fatokun, Annie Chimphango, Richard Bayitse, Paula Helena Blanco Sanchez, Patricia Thornley, Alfred Fernandez-Castane^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.

Original language	English
Article number	111815
Number of pages	13
Journal	Journal of Environmental Chemical engineering
Volume	12
Issue number	1
Early online date	25 Dec 2023
DOIs	https://doi.org/10.1016/j.jece.2023.111815
Publication status	Published - Feb 2024

Bibliographical note

This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords

Acid hydrolysis
Cassava
Cupriavidus necator
Polyhydroxyalkanoates
Waste valorization

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1-s2.0-S221334372302554X-main
This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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title = "Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates",

abstract = "Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.",

keywords = "Acid hydrolysis, Cassava, Cupriavidus necator, Polyhydroxyalkanoates, Waste valorization",

author = "Carmen Hierro-Iglesias and Fatokun, {Cornelius O.} and Annie Chimphango and Richard Bayitse and {Blanco Sanchez}, {Paula Helena} and Patricia Thornley and Alfred Fernandez-Castane",

note = "This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.",

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AU - Hierro-Iglesias, Carmen

AU - Fatokun, Cornelius O.

AU - Chimphango, Annie

AU - Bayitse, Richard

AU - Blanco Sanchez, Paula Helena

AU - Thornley, Patricia

AU - Fernandez-Castane, Alfred

N1 - This is an open access article distributed under the terms of the Creative Commons Attribution License CC BY [https://creativecommons.org/licenses/by/4.0/], which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PY - 2024/2

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N2 - Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.

AB - Polyhydroxyalkanoates (PHA) are biodegradable polyesters that can be produced from renewable resources. However, PHA biomanufacturing is costly compared to petrochemical-based plastics. A promising solution consists of using cassava (Manhiot esculenta) waste, abundant biomass in developing countries, as a carbon source for PHA production. This study involved characterising untreated and acid-hydrolysed cassava peel (CP) to confirm the degradation of polysaccharides into fermentable sugars after pre-treatment. A chemical and biological integrated process was developed, optimising the pre-treatment using a central composite design. The highest conversion of CP into reducing sugars was 97% (w/w) using 3 M H2SO4, 120 min and 90 ºC. The ability of Cupriavidus necator to grow on CP hydrolysate and produce PHA was screened resulting in up to OD600 15.8 and 1.5 g/L of PHA (31% (gPHA/gDCW)). Flow cytometry allowed rapid, simple, and high-throughput assessment of PHA content. These findings pave the way for developing a biorefinery platform for PHA production from cassava waste.

KW - Acid hydrolysis

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KW - Cupriavidus necator

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KW - Waste valorization

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Process integration for efficient conversion of cassava peel waste into polyhydroxyalkanoates

Abstract

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Keywords

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