Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons

Thomas D.P. Allsop; Chengbo Mou; Ron Neal; Stefano Mariani; David Nagel; Sara Tombelli; Andrew Poole; Kyriacos Kalli; Anna V.  Hine; David J. Webb; Philip Culverhouse; M. Mascini; Maria Minunni; Ian Bennion

doi:10.1364/OE.25.000039

Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons

Thomas D.P. Allsop^*, Chengbo Mou, Ron Neal, Stefano Mariani, David Nagel, Sara Tombelli, Andrew Poole, Kyriacos Kalli, Anna V. Hine, David J. Webb, Philip Culverhouse, M. Mascini, Maria Minunni, Ian Bennion

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Here we present a new generic opto-bio-sensing platform combining immobilised aptamers on an infrared plasmonic sensing device generated by nano-structured thin film that demonstrates amongst the highest index spectral sensitivities of any optical fibre sensor yielding on average 3.4 × 10⁴ nm/RIU in the aqueous index regime (with a figure of merit of 330) This offers a single stage, solution phase, atto-molar detection capability, whilst delivering real-time data for kinetic studies in water-based chemistry. The sensing platform is based upon optical fibre and has the potential to be multiplexed and used in remote sensing applications. As an example of the highly versatile capabilities of aptamer based detection using our platform, purified thrombin is detected down to 50 attomolar concentration using a volume of 1mm³ of solution without the use of any form of enhancement technique. Moreover, the device can detect nanomolar levels of thrombin in a flow cell, in the presence of 4.5% w/v albumin solution. These results are important, covering all concentrations in the human thrombin generation curve, including the problematic initial phase. Finally, selectivity is confirmed using complementary and non-complementary DNA sequences that yield performances similar to those obtained with thrombin.

Original language	English
Pages (from-to)	39-58
Number of pages	20
Journal	Optics Express
Volume	25
Issue number	1
Early online date	3 Jan 2017
DOIs	https://doi.org/10.1364/OE.25.000039
Publication status	Published - 9 Jan 2017

Bibliographical note

© 2017 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.

Funding: EPSRC (EP/J010413 and EP/J010391)

Access to Document

10.1364/OE.25.000039

Real-time kinetic binding studies at attomolar concentrations in solution phase
© 2017 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
Accepted author manuscript, 1.89 MB

Cite this

Allsop, T. D. P., Mou, C., Neal, R., Mariani, S., Nagel, D., Tombelli, S., Poole, A., Kalli, K., Hine, A. V., Webb, D. J., Culverhouse, P., Mascini, M., Minunni, M., & Bennion, I. (2017). Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons. Optics Express, 25(1), 39-58. https://doi.org/10.1364/OE.25.000039

@article{b4298e41a5b64957901945166516d8fd,

title = "Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons",

abstract = "Here we present a new generic opto-bio-sensing platform combining immobilised aptamers on an infrared plasmonic sensing device generated by nano-structured thin film that demonstrates amongst the highest index spectral sensitivities of any optical fibre sensor yielding on average 3.4 × 104 nm/RIU in the aqueous index regime (with a figure of merit of 330) This offers a single stage, solution phase, atto-molar detection capability, whilst delivering real-time data for kinetic studies in water-based chemistry. The sensing platform is based upon optical fibre and has the potential to be multiplexed and used in remote sensing applications. As an example of the highly versatile capabilities of aptamer based detection using our platform, purified thrombin is detected down to 50 attomolar concentration using a volume of 1mm3 of solution without the use of any form of enhancement technique. Moreover, the device can detect nanomolar levels of thrombin in a flow cell, in the presence of 4.5% w/v albumin solution. These results are important, covering all concentrations in the human thrombin generation curve, including the problematic initial phase. Finally, selectivity is confirmed using complementary and non-complementary DNA sequences that yield performances similar to those obtained with thrombin.",

author = "Allsop, {Thomas D.P.} and Chengbo Mou and Ron Neal and Stefano Mariani and David Nagel and Sara Tombelli and Andrew Poole and Kyriacos Kalli and Hine, {Anna V.} and Webb, {David J.} and Philip Culverhouse and M. Mascini and Maria Minunni and Ian Bennion",

note = "{\textcopyright} 2017 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited. Funding: EPSRC (EP/J010413 and EP/J010391) ",

year = "2017",

month = jan,

day = "9",

doi = "10.1364/OE.25.000039",

language = "English",

volume = "25",

pages = "39--58",

journal = "Optics Express",

issn = "1094-4087",

publisher = "The Optical Society",

number = "1",

}

Allsop, TDP, Mou, C, Neal, R, Mariani, S, Nagel, D, Tombelli, S, Poole, A, Kalli, K, Hine, AV , Webb, DJ, Culverhouse, P, Mascini, M, Minunni, M & Bennion, I 2017, 'Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons', Optics Express, vol. 25, no. 1, pp. 39-58. https://doi.org/10.1364/OE.25.000039

TY - JOUR

T1 - Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons

AU - Allsop, Thomas D.P.

AU - Mou, Chengbo

AU - Neal, Ron

AU - Mariani, Stefano

AU - Nagel, David

AU - Tombelli, Sara

AU - Poole, Andrew

AU - Kalli, Kyriacos

AU - Hine, Anna V.

AU - Webb, David J.

AU - Culverhouse, Philip

AU - Mascini, M.

AU - Minunni, Maria

AU - Bennion, Ian

N1 - © 2017 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited. Funding: EPSRC (EP/J010413 and EP/J010391)

PY - 2017/1/9

Y1 - 2017/1/9

N2 - Here we present a new generic opto-bio-sensing platform combining immobilised aptamers on an infrared plasmonic sensing device generated by nano-structured thin film that demonstrates amongst the highest index spectral sensitivities of any optical fibre sensor yielding on average 3.4 × 104 nm/RIU in the aqueous index regime (with a figure of merit of 330) This offers a single stage, solution phase, atto-molar detection capability, whilst delivering real-time data for kinetic studies in water-based chemistry. The sensing platform is based upon optical fibre and has the potential to be multiplexed and used in remote sensing applications. As an example of the highly versatile capabilities of aptamer based detection using our platform, purified thrombin is detected down to 50 attomolar concentration using a volume of 1mm3 of solution without the use of any form of enhancement technique. Moreover, the device can detect nanomolar levels of thrombin in a flow cell, in the presence of 4.5% w/v albumin solution. These results are important, covering all concentrations in the human thrombin generation curve, including the problematic initial phase. Finally, selectivity is confirmed using complementary and non-complementary DNA sequences that yield performances similar to those obtained with thrombin.

AB - Here we present a new generic opto-bio-sensing platform combining immobilised aptamers on an infrared plasmonic sensing device generated by nano-structured thin film that demonstrates amongst the highest index spectral sensitivities of any optical fibre sensor yielding on average 3.4 × 104 nm/RIU in the aqueous index regime (with a figure of merit of 330) This offers a single stage, solution phase, atto-molar detection capability, whilst delivering real-time data for kinetic studies in water-based chemistry. The sensing platform is based upon optical fibre and has the potential to be multiplexed and used in remote sensing applications. As an example of the highly versatile capabilities of aptamer based detection using our platform, purified thrombin is detected down to 50 attomolar concentration using a volume of 1mm3 of solution without the use of any form of enhancement technique. Moreover, the device can detect nanomolar levels of thrombin in a flow cell, in the presence of 4.5% w/v albumin solution. These results are important, covering all concentrations in the human thrombin generation curve, including the problematic initial phase. Finally, selectivity is confirmed using complementary and non-complementary DNA sequences that yield performances similar to those obtained with thrombin.

UR - https://www.osapublishing.org/oe/abstract.cfm?uri=oe-25-1-39

UR - http://doi.org/10.17036/researchdata.aston.ac.uk.00000179

UR - http://www.scopus.com/inward/record.url?scp=85009517271&partnerID=8YFLogxK

U2 - 10.1364/OE.25.000039

DO - 10.1364/OE.25.000039

M3 - Article

SN - 1094-4087

VL - 25

SP - 39

EP - 58

JO - Optics Express

JF - Optics Express

IS - 1

ER -

Real-time kinetic binding studies at attomolar concentrations in solution phase using a single-stage opto-biosensing platform based upon infrared surface plasmons

Abstract

Bibliographical note

Access to Document

Other files and links

Fingerprint