Abstract
We report the first demonstration of real-time biosensing in free standing macroporous alumina membranes. The membranes with their 200 nm diameter pores are ideal candidates for biosensing applications where fast response times for small sample volumes are needed as they allow analytes to flow through the pores close to the bioreceptors immobilized on the pores walls. A bulk refractive index sensitivity of 5.2×10-6 refractive index units was obtained from signal responses to different concentrations of NaCl solutions flowing through the pores. Finally, after functionalizing the alumina pore surfaces with an epoxysilane and then spotting it with β-Lactoglobulin protein, the interactions between the β-lactoglobulin and rabbit anti-β-lactoglobulin, as well as the interaction between the rabbit anti-β-lactoglobulin and a secondary antibody anti-rabbit Immunoglobulin G were monitored in real-time.
| Original language | English |
|---|---|
| Article number | 87650I |
| Journal | Proceedings of SPIE - International Society for Optical Engineering |
| Volume | 8765 |
| DOIs | |
| Publication status | Published - 28 May 2013 |
| Event | SPIE Microtechnologies BioMEMS and Medical Microdevices Conference - Grenoble, France Duration: 25 Apr 2013 → 26 Apr 2013 |
Bibliographical note
Copyright 2013 SPIE. One print or electronic copy may be made for personal use only. Systematic reproduction, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.Keywords
- Biological sensing and sensors
- Form birefringence
- Optical sensing and sensors
- Polarimetry
- Porous alumina