Abstract
Fluorophore 1,8-naphthilamide was linked to 2-bromoacridine through an ethylenediamine spacer using a succinct synthetic route to give a bromoacridine-linked bifunctional fluorophore conjugate for the detection of triplex DNA. Acridine is well known to intercalate into duplex DNA whereas introduction of a bulky bromine atom at position C2 redirects specificity for triplex over duplex DNA. In this work, photoelectron transfer assay was used to demonstrate that the synthesised 2-bromoacridine-linked fluorophore conjugate had good selectivity for the representative triplex DNA target sequence d(T*A.T)20 compared with double-stranded d(T.A)20, single-stranded dT20 or d(G/A)19 DNA sequences.
| Original language | English |
|---|---|
| Pages (from-to) | 1013-1016 |
| Number of pages | 4 |
| Journal | Monatshefte für Chemier |
| Volume | 152 |
| Issue number | 8 |
| Early online date | 7 Aug 2021 |
| DOIs | |
| Publication status | Published - Aug 2021 |
Bibliographical note
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- Bisensors
- Fluorescence spectroscopy
- Heterocycles
- Nucleic acids