Serological detection of Gram-positive bacterial infection around prostheses

M. Rafiq; T. Worthington; S. E. Tebbs; R. B.C. Treacy; R. Dias; P. A. Lambert; T. S.J. Elliott

doi:10.1302/0301-620X.82B8.10366

Serological detection of Gram-positive bacterial infection around prostheses

M. Rafiq, T. Worthington, S. E. Tebbs, R. B.C. Treacy, R. Dias, P. A. Lambert, T. S.J. Elliott^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Coagulase-negative staphylococci produce an exocellular glycolipid antigen which has potential as a serological marker of infection in bone. The value of this newly detected antigen was investigated by enzyme-linked immunosorbent assay (ELISA) in 15 patients with culture-proven infection of prostheses caused by Gram-positive bacteria. The antigen was purified by gel-permeation chromatography from the culture supernatants of coagulase-negative staphylococci grown in a chemically defined medium. There were significant differences (p < 0.0001) between the serum IgG and IgM levels in patients with infection due to Gram-positive staphylococci and those of a control group of 32 patients with no infection. The ELISA test, which has potential for the diagnosis of infection, may be valuable in distinguishing between staphylococcal infection around prostheses and aseptic loosening.

Original language	English
Pages (from-to)	1156-1161
Number of pages	6
Journal	Journal of Bone and Joint Surgery - Series B
Volume	82-B
Issue number	8
DOIs	https://doi.org/10.1302/0301-620X.82B8.10366
Publication status	Published - 1 Nov 2000

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10.1302/0301-620X.82B8.10366

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title = "Serological detection of Gram-positive bacterial infection around prostheses",

abstract = "Coagulase-negative staphylococci produce an exocellular glycolipid antigen which has potential as a serological marker of infection in bone. The value of this newly detected antigen was investigated by enzyme-linked immunosorbent assay (ELISA) in 15 patients with culture-proven infection of prostheses caused by Gram-positive bacteria. The antigen was purified by gel-permeation chromatography from the culture supernatants of coagulase-negative staphylococci grown in a chemically defined medium. There were significant differences (p < 0.0001) between the serum IgG and IgM levels in patients with infection due to Gram-positive staphylococci and those of a control group of 32 patients with no infection. The ELISA test, which has potential for the diagnosis of infection, may be valuable in distinguishing between staphylococcal infection around prostheses and aseptic loosening.",

author = "M. Rafiq and T. Worthington and Tebbs, {S. E.} and Treacy, {R. B.C.} and R. Dias and Lambert, {P. A.} and Elliott, {T. S.J.}",

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T1 - Serological detection of Gram-positive bacterial infection around prostheses

AU - Rafiq, M.

AU - Worthington, T.

AU - Tebbs, S. E.

AU - Treacy, R. B.C.

AU - Dias, R.

AU - Lambert, P. A.

AU - Elliott, T. S.J.

PY - 2000/11/1

Y1 - 2000/11/1

N2 - Coagulase-negative staphylococci produce an exocellular glycolipid antigen which has potential as a serological marker of infection in bone. The value of this newly detected antigen was investigated by enzyme-linked immunosorbent assay (ELISA) in 15 patients with culture-proven infection of prostheses caused by Gram-positive bacteria. The antigen was purified by gel-permeation chromatography from the culture supernatants of coagulase-negative staphylococci grown in a chemically defined medium. There were significant differences (p < 0.0001) between the serum IgG and IgM levels in patients with infection due to Gram-positive staphylococci and those of a control group of 32 patients with no infection. The ELISA test, which has potential for the diagnosis of infection, may be valuable in distinguishing between staphylococcal infection around prostheses and aseptic loosening.

AB - Coagulase-negative staphylococci produce an exocellular glycolipid antigen which has potential as a serological marker of infection in bone. The value of this newly detected antigen was investigated by enzyme-linked immunosorbent assay (ELISA) in 15 patients with culture-proven infection of prostheses caused by Gram-positive bacteria. The antigen was purified by gel-permeation chromatography from the culture supernatants of coagulase-negative staphylococci grown in a chemically defined medium. There were significant differences (p < 0.0001) between the serum IgG and IgM levels in patients with infection due to Gram-positive staphylococci and those of a control group of 32 patients with no infection. The ELISA test, which has potential for the diagnosis of infection, may be valuable in distinguishing between staphylococcal infection around prostheses and aseptic loosening.

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UR - https://online.boneandjoint.org.uk/doi/abs/10.1302/0301-620X.82B8.0821156

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SP - 1156

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JO - Journal of Bone and Joint Surgery - Series B

JF - Journal of Bone and Joint Surgery - Series B

IS - 8

ER -

Serological detection of Gram-positive bacterial infection around prostheses

Abstract

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