Stimulation of adenylate cyclase by amylin in CHO-K1 cells

C.S. D'Santos, A. Gatti, David R. Poyner, Michael R. Hanley

Research output: Contribution to journalArticlepeer-review


The CHO-K1 cell line responds to the peptide amylin by a rapid elevation of cAMP. The related peptide calcitonin gene-related peptide (CGRP) is 100 times less potent at stimulating adenylate cyclase than is amylin. The actions of amylin at this receptor are concentration dependent and not antagonized by the CGRP antagonist CGRP-(8-37). Although these cells have receptors for calcitonin, amylin is unable to take part in any high affinity interaction with these receptors, as assessed by radioligand binding. The CHO-K1 cell line has receptors for amylin that are distinct from those for calcitonin and CGRP.
Original languageEnglish
Pages (from-to)894-899
Number of pages6
JournalMolecular Pharmacology
Issue number5
Publication statusPublished - 1 May 1992


  • 1-Methyl-3-isobutylxanthine
  • adenylate
  • cyclase
  • amyloid
  • animals
  • CHO
  • cells
  • calcitonin
  • gene-related peptide
  • cricetinae cyclic
  • AMP
  • dose-response relationship
  • drug
  • enzyme activation
  • humans kinetics receptors
  • calcitonin receptors
  • cell surface


Dive into the research topics of 'Stimulation of adenylate cyclase by amylin in CHO-K1 cells'. Together they form a unique fingerprint.

Cite this