Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry

Andrew D. Palmer; Rian Griffiths; Iain Styles; Ela Claridge; Antonio Calcagni; Josephine Bunch

doi:10.1002/jms.2049

Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry

Andrew D. Palmer, Rian Griffiths, Iain Styles, Ela Claridge, Antonio Calcagni, Josephine Bunch^*

^*Corresponding author for this work

Optometry

Research output: Contribution to journal › Article › peer-review

Abstract

Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-μm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections.

Original language	English
Pages (from-to)	237-241
Number of pages	5
Journal	Journal of Mass Spectrometry
Volume	47
Issue number	2
DOIs	https://doi.org/10.1002/jms.2049
Publication status	Published - Feb 2012

Keywords

cryo-protection
eye
histology
imaging
MALDI
sucrose

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10.1002/jms.2049

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abstract = "Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-μm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections.",

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AU - Griffiths, Rian

AU - Styles, Iain

AU - Claridge, Ela

AU - Calcagni, Antonio

AU - Bunch, Josephine

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AB - Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-μm thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections.

KW - cryo-protection

KW - eye

KW - histology

KW - imaging

KW - MALDI

KW - sucrose

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Sucrose cryo-protection facilitates imaging of whole eye sections by MALDI mass spectrometry

Abstract

Keywords

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