The development of attenuation compensation models of fluorescence spectroscopy signals

Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova; Andrey V. Dunaev

doi:10.1117/12.2229451

The development of attenuation compensation models of fluorescence spectroscopy signals

Victor V. Dremin, Evgeny A. Zherebtsov, Ilya E. Rafailov, Andrey Y. Vinokurov, Irina N. Novikova, Angelina I. Zherebtsova, Karina S. Litvinova, Andrey V. Dunaev

College of Engineering and Physical Sciences

Research output: Chapter in Book/Published conference output › Conference publication

Abstract

This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry. We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra. In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.

Original language	English
Title of host publication	Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)
Editors	Elina A. Genina, Valery V. Tuchin, Vladimir L. Derbov, et al
Place of Publication	Bellingham, WA (US)
Publisher	SPIE
Number of pages	6
ISBN (Print)	978-1-5106-0226-7
DOIs	https://doi.org/10.1117/12.2229451
Publication status	Published - 21 Apr 2016
Event	3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015 - Saratov, Russian Federation Duration: 22 Sept 2015 → 25 Sept 2015

Publication series

Name	SPIE Proceedings
Publisher	SPIE
Volume	9917
ISSN (Print)	0277-786X
ISSN (Electronic)	2410-9045

Conference

Conference	3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015
Country/Territory	Russian Federation
City	Saratov
Period	22/09/15 → 25/09/15

Bibliographical note

-Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova and Andrey V. Dunaev, "The development of attenuation compensation models of fluorescence spectroscopy signals", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (April 21, 2016).

Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

DOI: http://dx.doi.org/10.1117/12.2229451

Keywords

3D model
correction spectra
diffuse reflectance spectroscopy
fluorescence spectroscopy
laser Doppler flowmetry
Monte Carlo simulation

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10.1117/12.2229451

Attenuation compensation models of fluorescence spectroscopy signals
Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova and Andrey V. Dunaev, "The development of attenuation compensation models of fluorescence spectroscopy signals", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (April 21, 2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2229451
Final published version, 768 KB

http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=2516953

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Dremin, V. V., Zherebtsov, E. A., Rafailov, I. E., Vinokurov, A. Y., Novikova, I. N., Zherebtsova, A. I., Litvinova, K. S., & Dunaev, A. V. (2016). The development of attenuation compensation models of fluorescence spectroscopy signals. In E. A. Genina, V. V. Tuchin, V. L. Derbov, & et al (Eds.), Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS) Article 99170Y (SPIE Proceedings; Vol. 9917). SPIE. https://doi.org/10.1117/12.2229451

Dremin, Victor V. ; Zherebtsov, Evgeny A. ; Rafailov, Ilya E. et al. / The development of attenuation compensation models of fluorescence spectroscopy signals. Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS). editor / Elina A. Genina ; Valery V. Tuchin ; Vladimir L. Derbov ; et al. Bellingham, WA (US) : SPIE, 2016. (SPIE Proceedings).

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title = "The development of attenuation compensation models of fluorescence spectroscopy signals",

abstract = "This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry. We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra. In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.",

keywords = "3D model, correction spectra, diffuse reflectance spectroscopy, fluorescence spectroscopy, laser Doppler flowmetry, Monte Carlo simulation",

author = "Dremin, {Victor V.} and Zherebtsov, {Evgeny A.} and Rafailov, {Ilya E.} and Vinokurov, {Andrey Y.} and Novikova, {Irina N.} and Zherebtsova, {Angelina I.} and Litvinova, {Karina S.} and Dunaev, {Andrey V.}",

note = "-Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova and Andrey V. Dunaev, {"}The development of attenuation compensation models of fluorescence spectroscopy signals{"}, Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (April 21, 2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2229451; 3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015 ; Conference date: 22-09-2015 Through 25-09-2015",

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editor = "Genina, {Elina A.} and Tuchin, {Valery V.} and Derbov, {Vladimir L.} and {et al}",

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Dremin, VV, Zherebtsov, EA, Rafailov, IE, Vinokurov, AY, Novikova, IN, Zherebtsova, AI, Litvinova, KS & Dunaev, AV 2016, The development of attenuation compensation models of fluorescence spectroscopy signals. in EA Genina, VV Tuchin, VL Derbov & et al (eds), Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)., 99170Y, SPIE Proceedings, vol. 9917, SPIE, Bellingham, WA (US), 3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015, Saratov, Russian Federation, 22/09/15. https://doi.org/10.1117/12.2229451

The development of attenuation compensation models of fluorescence spectroscopy signals. / Dremin, Victor V.; Zherebtsov, Evgeny A.; Rafailov, Ilya E. et al.
Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS). ed. / Elina A. Genina; Valery V. Tuchin; Vladimir L. Derbov; et al. Bellingham, WA (US): SPIE, 2016. 99170Y (SPIE Proceedings; Vol. 9917).

Research output: Chapter in Book/Published conference output › Conference publication

TY - GEN

T1 - The development of attenuation compensation models of fluorescence spectroscopy signals

AU - Dremin, Victor V.

AU - Zherebtsov, Evgeny A.

AU - Rafailov, Ilya E.

AU - Vinokurov, Andrey Y.

AU - Novikova, Irina N.

AU - Zherebtsova, Angelina I.

AU - Litvinova, Karina S.

AU - Dunaev, Andrey V.

N1 - -Victor V. Dremin; Evgeny A. Zherebtsov; Ilya E. Rafailov; Andrey Y. Vinokurov; Irina N. Novikova; Angelina I. Zherebtsova; Karina S. Litvinova and Andrey V. Dunaev, "The development of attenuation compensation models of fluorescence spectroscopy signals", Proc. SPIE 9917, Saratov Fall Meeting 2015: Third International Symposium on Optics and Biophotonics and Seventh Finnish-Russian Photonics and Laser Symposium (PALS), 99170Y (April 21, 2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. DOI: http://dx.doi.org/10.1117/12.2229451

PY - 2016/4/21

Y1 - 2016/4/21

N2 - This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry. We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra. In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.

AB - This study examines the effect of blood absorption on the endogenous fluorescence signal intensity of biological tissues. Experimental studies were conducted to identify these effects. To register the fluorescence intensity, the fluorescence spectroscopy method was employed. The intensity of the blood flow was measured by laser Doppler flowmetry. We proposed one possible implementation of the Monte Carlo method for the theoretical analysis of the effect of blood on the fluorescence signals. The simulation is constructed as a four-layer skin optical model based on the known optical parameters of the skin with different levels of blood supply. With the help of the simulation, we demonstrate how the level of blood supply can affect the appearance of the fluorescence spectra. In addition, to describe the properties of biological tissue, which may affect the fluorescence spectra, we turned to the method of diffuse reflectance spectroscopy (DRS). Using the spectral data provided by the DRS, the tissue attenuation effect can be extracted and used to correct the fluorescence spectra.

KW - 3D model

KW - correction spectra

KW - diffuse reflectance spectroscopy

KW - fluorescence spectroscopy

KW - laser Doppler flowmetry

KW - Monte Carlo simulation

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U2 - 10.1117/12.2229451

DO - 10.1117/12.2229451

M3 - Conference publication

AN - SCOPUS:84975073291

SN - 978-1-5106-0226-7

T3 - SPIE Proceedings

BT - Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS)

A2 - Genina, Elina A.

A2 - Tuchin, Valery V.

A2 - Derbov, Vladimir L.

A2 - et al,

PB - SPIE

CY - Bellingham, WA (US)

T2 - 3rd International Symposium on Optics and Biophotonics, SFM 2015 and 7th Finnish-Russian Photonics and Laser Symposium, PALS 2015

Y2 - 22 September 2015 through 25 September 2015

ER -

Dremin VV, Zherebtsov EA, Rafailov IE, Vinokurov AY, Novikova IN, Zherebtsova AI et al. The development of attenuation compensation models of fluorescence spectroscopy signals. In Genina EA, Tuchin VV, Derbov VL, et al, editors, Third International Symposium on Optics and Biophotonics; and Seventh Finnish-Russian Photonics and Laser Symposium (PALS). Bellingham, WA (US): SPIE. 2016. 99170Y. (SPIE Proceedings). doi: 10.1117/12.2229451

The development of attenuation compensation models of fluorescence spectroscopy signals

Abstract

Publication series

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Bibliographical note

Keywords

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