The phytochrome control of the cAMP endogenous level in oat seedlings

O. V. Molchan; S. G. Sokolovsky; I. D. Volotovsky

The phytochrome control of the cAMP endogenous level in oat seedlings

O. V. Molchan^*, S. G. Sokolovsky, I. D. Volotovsky

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The activity of adenylate cyclase (AC, EC 4.6.1.1) was studied in oat (Avena sativa L.) seedlings. The following conditions were optimal for AC activity: a substrate (Mg²⁺-ATP) concentration of 0.75 mM; a temperature of 20-25°C, and pH 7.5. AC activity was detected in all subcellular fractions. The highest activity was observed in the microsomal fraction. GTP, its nonhydrolyzable analogs, and forskolin substantially enhanced the AC activity, whereas extracellular Ca²⁺ had no effect. The AC is suggested to exist in plant tissues in a membrane-bound form. The endogenous cAMP level was shown to depend on seedling illumination: it was far lower in etiolated seedlings and those adapted to darkness. Red and far-red light affected the endogenous cAMP content, indicating the possible involvement of cAMP in the phytochrome signaling pathway.

Original language	English
Pages (from-to)	463-467
Number of pages	5
Journal	Russian Journal of Plant Physiology
Volume	47
Issue number	4
Publication status	Published - Jul 2000

Bibliographical note

Copyright:
Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.

Keywords

Adenylate cyclase activity
Avena sativa
cAMP
Phytochrome
Regulation
Seedlings

Cite this

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title = "The phytochrome control of the cAMP endogenous level in oat seedlings",

abstract = "The activity of adenylate cyclase (AC, EC 4.6.1.1) was studied in oat (Avena sativa L.) seedlings. The following conditions were optimal for AC activity: a substrate (Mg2+-ATP) concentration of 0.75 mM; a temperature of 20-25°C, and pH 7.5. AC activity was detected in all subcellular fractions. The highest activity was observed in the microsomal fraction. GTP, its nonhydrolyzable analogs, and forskolin substantially enhanced the AC activity, whereas extracellular Ca2+ had no effect. The AC is suggested to exist in plant tissues in a membrane-bound form. The endogenous cAMP level was shown to depend on seedling illumination: it was far lower in etiolated seedlings and those adapted to darkness. Red and far-red light affected the endogenous cAMP content, indicating the possible involvement of cAMP in the phytochrome signaling pathway.",

keywords = "Adenylate cyclase activity, Avena sativa, cAMP, Phytochrome, Regulation, Seedlings",

author = "Molchan, {O. V.} and Sokolovsky, {S. G.} and Volotovsky, {I. D.}",

year = "2000",

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language = "English",

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TY - JOUR

T1 - The phytochrome control of the cAMP endogenous level in oat seedlings

AU - Molchan, O. V.

AU - Sokolovsky, S. G.

AU - Volotovsky, I. D.

PY - 2000/7

Y1 - 2000/7

N2 - The activity of adenylate cyclase (AC, EC 4.6.1.1) was studied in oat (Avena sativa L.) seedlings. The following conditions were optimal for AC activity: a substrate (Mg2+-ATP) concentration of 0.75 mM; a temperature of 20-25°C, and pH 7.5. AC activity was detected in all subcellular fractions. The highest activity was observed in the microsomal fraction. GTP, its nonhydrolyzable analogs, and forskolin substantially enhanced the AC activity, whereas extracellular Ca2+ had no effect. The AC is suggested to exist in plant tissues in a membrane-bound form. The endogenous cAMP level was shown to depend on seedling illumination: it was far lower in etiolated seedlings and those adapted to darkness. Red and far-red light affected the endogenous cAMP content, indicating the possible involvement of cAMP in the phytochrome signaling pathway.

AB - The activity of adenylate cyclase (AC, EC 4.6.1.1) was studied in oat (Avena sativa L.) seedlings. The following conditions were optimal for AC activity: a substrate (Mg2+-ATP) concentration of 0.75 mM; a temperature of 20-25°C, and pH 7.5. AC activity was detected in all subcellular fractions. The highest activity was observed in the microsomal fraction. GTP, its nonhydrolyzable analogs, and forskolin substantially enhanced the AC activity, whereas extracellular Ca2+ had no effect. The AC is suggested to exist in plant tissues in a membrane-bound form. The endogenous cAMP level was shown to depend on seedling illumination: it was far lower in etiolated seedlings and those adapted to darkness. Red and far-red light affected the endogenous cAMP content, indicating the possible involvement of cAMP in the phytochrome signaling pathway.

KW - Adenylate cyclase activity

KW - Avena sativa

KW - cAMP

KW - Phytochrome

KW - Regulation

KW - Seedlings

UR - http://www.scopus.com/inward/record.url?scp=0034342126&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0034342126

SN - 1021-4437

VL - 47

SP - 463

EP - 467

JO - Russian Journal of Plant Physiology

JF - Russian Journal of Plant Physiology

IS - 4

ER -

The phytochrome control of the cAMP endogenous level in oat seedlings

Abstract

Bibliographical note

Keywords

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