Titanium phosphate glass microspheres for bone tissue engineering

Nilay J. Lakhkar; Jeong-Hui Park; Nicola J. Mordan; Vehid Salih; Ivan B. Wall; Hae-Won Kim; Scott P. King; John V. Hanna; Richard A. Martin; Owen Addison; J.F.W. Mosselmans; Jonathan C. Knowles

doi:10.1016/j.actbio.2012.07.023

Titanium phosphate glass microspheres for bone tissue engineering

Nilay J. Lakhkar, Jeong-Hui Park, Nicola J. Mordan, Vehid Salih, Ivan B. Wall, Hae-Won Kim, Scott P. King, John V. Hanna, Richard A. Martin, Owen Addison, J.F.W. Mosselmans, Jonathan C. Knowles

School of Biosciences

Research output: Contribution to journal › Article › peer-review

Abstract

We have demonstrated the successful production of titanium phosphate glass microspheres in the size range of ~10-200 µm using an inexpensive, efficient, easily scalable process and assessed their use in bone tissue engineering applications. Glasses of the following compositions were prepared by melt-quench techniques: 0.5P₂O₅-0.4CaO-(0.1 - x)Na₂O-xTiO₂, where x = 0.03, 0.05 and 0.07 mol fraction (denoted as Ti3, Ti5 and Ti7 respectively). Several characterization studies such as differential thermal analysis, degradation (performed using a novel time lapse imaging technique) and pH and ion release measurements revealed significant densification of the glass structure with increased incorporation of TiO₂ in the glass from 3 to 5 mol.%, although further TiO₂ incorporation up to 7 mol.% did not affect the glass structure to the same extent. Cell culture studies performed using MG63 cells over a 7-day period clearly showed the ability of the microspheres to provide a stable surface for cell attachment, growth and proliferation. Taken together, the results confirm that 5 mol.% TiO₂ glass microspheres, on account of their relative ease of preparation and favourable biocompatibility, are worthy candidates for use as substrate materials in bone tissue engineering applications.

Original language	English
Pages (from-to)	4181-4190
Number of pages	10
Journal	Acta Biomaterialia
Volume	8
Issue number	11
Early online date	23 Jul 2012
DOIs	https://doi.org/10.1016/j.actbio.2012.07.023
Publication status	Published - Nov 2012

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Acta biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Lakhkar, N. J., Park, J-H., Mordan, N. J., Salih, V., Wall, I. B., Kim, H-W., King, S. P., Hanna, J. V. , Martin, R. A., Addison, O., Mosselmans, J. F. W., & Knowles, J. C. (2012). Titanium phosphate glass microspheres for bone tissue engineering. Acta biomaterialia, vol. 8, No. 11 (2012) DOI http://dx.doi.org/10.1016/j.actbio.2012.07.023

Keywords

titanium
phosphate glass
microspheres
bone cells
tissue engineering

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10.1016/j.actbio.2012.07.023

Titanium phosphate glass microspheres for bone tissue engineering
NOTICE: this is the author’s version of a work that was accepted for publication in Acta biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Lakhkar, N. J., Park, J-H., Mordan, N. J., Salih, V., Wall, I. B., Kim, H-W., King, S. P., Hanna, J. V. , Martin, R. A., Addison, O., Mosselmans, J. F. W., & Knowles, J. C. (2012). Titanium phosphate glass microspheres for bone tissue engineering. Acta biomaterialia, vol. 8, No. 11 (2012) DOI http://dx.doi.org/10.1016/j.actbio.2012.07.023
Accepted author manuscript, 652 KB

Cite this

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title = "Titanium phosphate glass microspheres for bone tissue engineering",

abstract = "We have demonstrated the successful production of titanium phosphate glass microspheres in the size range of ~10-200 µm using an inexpensive, efficient, easily scalable process and assessed their use in bone tissue engineering applications. Glasses of the following compositions were prepared by melt-quench techniques: 0.5P2O5-0.4CaO-(0.1 - x)Na2O-xTiO2, where x = 0.03, 0.05 and 0.07 mol fraction (denoted as Ti3, Ti5 and Ti7 respectively). Several characterization studies such as differential thermal analysis, degradation (performed using a novel time lapse imaging technique) and pH and ion release measurements revealed significant densification of the glass structure with increased incorporation of TiO2 in the glass from 3 to 5 mol.%, although further TiO2 incorporation up to 7 mol.% did not affect the glass structure to the same extent. Cell culture studies performed using MG63 cells over a 7-day period clearly showed the ability of the microspheres to provide a stable surface for cell attachment, growth and proliferation. Taken together, the results confirm that 5 mol.% TiO2 glass microspheres, on account of their relative ease of preparation and favourable biocompatibility, are worthy candidates for use as substrate materials in bone tissue engineering applications.",

keywords = "titanium, phosphate glass, microspheres, bone cells, tissue engineering",

author = "Lakhkar, {Nilay J.} and Jeong-Hui Park and Mordan, {Nicola J.} and Vehid Salih and Wall, {Ivan B.} and Hae-Won Kim and King, {Scott P.} and Hanna, {John V.} and Martin, {Richard A.} and Owen Addison and J.F.W. Mosselmans and Knowles, {Jonathan C.}",

note = "NOTICE: this is the author{\textquoteright}s version of a work that was accepted for publication in Acta biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Lakhkar, N. J., Park, J-H., Mordan, N. J., Salih, V., Wall, I. B., Kim, H-W., King, S. P., Hanna, J. V. , Martin, R. A., Addison, O., Mosselmans, J. F. W., & Knowles, J. C. (2012). Titanium phosphate glass microspheres for bone tissue engineering. Acta biomaterialia, vol. 8, No. 11 (2012) DOI http://dx.doi.org/10.1016/j.actbio.2012.07.023",

year = "2012",

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doi = "10.1016/j.actbio.2012.07.023",

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AU - Lakhkar, Nilay J.

AU - Park, Jeong-Hui

AU - Mordan, Nicola J.

AU - Salih, Vehid

AU - Wall, Ivan B.

AU - Kim, Hae-Won

AU - King, Scott P.

AU - Hanna, John V.

AU - Martin, Richard A.

AU - Addison, Owen

AU - Mosselmans, J.F.W.

AU - Knowles, Jonathan C.

N1 - NOTICE: this is the author’s version of a work that was accepted for publication in Acta biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Lakhkar, N. J., Park, J-H., Mordan, N. J., Salih, V., Wall, I. B., Kim, H-W., King, S. P., Hanna, J. V. , Martin, R. A., Addison, O., Mosselmans, J. F. W., & Knowles, J. C. (2012). Titanium phosphate glass microspheres for bone tissue engineering. Acta biomaterialia, vol. 8, No. 11 (2012) DOI http://dx.doi.org/10.1016/j.actbio.2012.07.023

PY - 2012/11

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N2 - We have demonstrated the successful production of titanium phosphate glass microspheres in the size range of ~10-200 µm using an inexpensive, efficient, easily scalable process and assessed their use in bone tissue engineering applications. Glasses of the following compositions were prepared by melt-quench techniques: 0.5P2O5-0.4CaO-(0.1 - x)Na2O-xTiO2, where x = 0.03, 0.05 and 0.07 mol fraction (denoted as Ti3, Ti5 and Ti7 respectively). Several characterization studies such as differential thermal analysis, degradation (performed using a novel time lapse imaging technique) and pH and ion release measurements revealed significant densification of the glass structure with increased incorporation of TiO2 in the glass from 3 to 5 mol.%, although further TiO2 incorporation up to 7 mol.% did not affect the glass structure to the same extent. Cell culture studies performed using MG63 cells over a 7-day period clearly showed the ability of the microspheres to provide a stable surface for cell attachment, growth and proliferation. Taken together, the results confirm that 5 mol.% TiO2 glass microspheres, on account of their relative ease of preparation and favourable biocompatibility, are worthy candidates for use as substrate materials in bone tissue engineering applications.

AB - We have demonstrated the successful production of titanium phosphate glass microspheres in the size range of ~10-200 µm using an inexpensive, efficient, easily scalable process and assessed their use in bone tissue engineering applications. Glasses of the following compositions were prepared by melt-quench techniques: 0.5P2O5-0.4CaO-(0.1 - x)Na2O-xTiO2, where x = 0.03, 0.05 and 0.07 mol fraction (denoted as Ti3, Ti5 and Ti7 respectively). Several characterization studies such as differential thermal analysis, degradation (performed using a novel time lapse imaging technique) and pH and ion release measurements revealed significant densification of the glass structure with increased incorporation of TiO2 in the glass from 3 to 5 mol.%, although further TiO2 incorporation up to 7 mol.% did not affect the glass structure to the same extent. Cell culture studies performed using MG63 cells over a 7-day period clearly showed the ability of the microspheres to provide a stable surface for cell attachment, growth and proliferation. Taken together, the results confirm that 5 mol.% TiO2 glass microspheres, on account of their relative ease of preparation and favourable biocompatibility, are worthy candidates for use as substrate materials in bone tissue engineering applications.

KW - titanium

KW - phosphate glass

KW - microspheres

KW - bone cells

KW - tissue engineering

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U2 - 10.1016/j.actbio.2012.07.023

DO - 10.1016/j.actbio.2012.07.023

M3 - Article

C2 - 22835676

SN - 1742-7061

VL - 8

SP - 4181

EP - 4190

JO - Acta Biomaterialia

JF - Acta Biomaterialia

IS - 11

ER -

Titanium phosphate glass microspheres for bone tissue engineering

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Keywords

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