Transcranial Photosensitizer-Free Laser Treatment of Glioblastoma in Rat Brain

Oxana Semyachkina-Glushkovskaya, Sergey Sokolovski, Ivan Fedosov, Alexander Shirokov, Nikita Navolokin, Alla Bucharskaya, Inna Blokhina, Andrey Terskov, Alexander Dubrovski, Valeria Telnova, Anna Tzven, Maria Tzoy, Arina Evsukova, Daria Zhlatogosrkaya, Viktoria Adushkina, Alexander Dmitrenko, Maria Manzhaeva, Valeria Krupnova, Alessio Noghero, Denis BraginOlga Bragina, Ekaterina Borisova, Jürgen Kurths, Edik Rafailov

Research output: Contribution to journalArticlepeer-review


Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly in neuroscience, neurodegenerative diseases and brain tumors. Glioblastoma is the most lethal form of brain cancer, with very limited treatment options and a poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free laser treatment (PS-free-LT) using a quantum-dot-based 1267 nm laser diode. This wavelength, highly absorbed by oxygen, is capable of turning triplet oxygen to singlet form. Applying 1267 nm laser irradiation for a 4 week course with a total dose of 12.7 kJ/cm2 firmly suppresses GBM growth and increases survival rate from 34% to 64%, presumably via LT-activated apoptosis, inhibition of the proliferation of tumor cells, a reduction in intracranial pressure and stimulation of the lymphatic drainage and clearing functions. PS-free-LT is a promising breakthrough technology in non- or minimally invasive therapy for superficial GBMs in infants as well as in adult patients with high photosensitivity or an allergic reaction to PSs.
Original languageEnglish
Article number13696
Number of pages25
JournalInternational Journal of Molecular Sciences
Issue number18
Early online date5 Sept 2023
Publication statusPublished - 5 Sept 2023

Bibliographical note

Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
This article is an open access article distributed under the terms and
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Funding: O.S.-G., I.F., A.S., N.N., I.B., A.T., A.D., M.Z., A.E., D.Z., V.A., A.D., M.M. and V.K. were supported by a grant (No. 23-75-30001) from the Russian Science Foundation; N.N., D.Z. and V.A. were supported by a grant (No. 21-75-10088) from the Russian Science Foundation; O.S.-G., I.F., A.S., N.N., I.B., A.T. and M.T. were supported by a grant from the Russian Ministry of Science and Higher
Education (No. 075-15-2022-1094); D.B. was supported by NIH R21NS091600 and R01 NS112808. E.R. and S.S. gratefully acknowledge funding from the HE Pathfinder GlioLight project (number 101129705). All cytological experiments, microscopy in the light field and fluorescence modes and electron microscopic examinations were carried out using equipment from the research equipment
center “SYMBIOSIS” within research project No. GR 121031100266-3 (IBPPM RAS).


  • glioblastoma
  • phototherapy
  • quantum-dot 1267 nm laser
  • NIR laser oxidative cell damaging
  • lymphatic system


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