TY - JOUR
T1 - Transcription of the plasmid-encoded toxin gene from enteroaggregative Escherichia coli is regulated by a novel co-activation mechanism involving CRP and Fis
AU - Rossiter, Amanda E
AU - Browning, Douglas F
AU - Leyton, Denisse L
AU - Johnson, Matthew D
AU - Godfrey, Rita E
AU - Wardius, Catherine A
AU - Desvaux, Mickael
AU - Cunningham, Adam F
AU - Ruiz-Perez, Fernando
AU - Nataro, James P
AU - Busby, Stephen J W
AU - Henderson, Ian R
PY - 2011/7
Y1 - 2011/7
N2 - Enteroaggregative Escherichia coli (EAEC) is a major cause of diarrhoea in developing countries. EAEC 042 is the prototypical strain. EAEC 042 secretes the functionally well-characterized Pet autotransporter toxin that contributes to virulence through its cytotoxic effects on intestinal epithelial cells. Following a global transposon mutagenesis screen of EAEC 042, the transcription factors, CRP and Fis, were identified as essential for transcription of the pet gene. Using both in vivo and in vitro techniques, we show that the pet promoter is co-dependent on CRP and Fis. We present a novel co-activation mechanism whereby CRP is placed at a non-optimal position for transcription initiation, creating dependence on Fis for full activation of pet. This study complements previous findings that establish Fis as a key virulence regulator in EAEC 042.
AB - Enteroaggregative Escherichia coli (EAEC) is a major cause of diarrhoea in developing countries. EAEC 042 is the prototypical strain. EAEC 042 secretes the functionally well-characterized Pet autotransporter toxin that contributes to virulence through its cytotoxic effects on intestinal epithelial cells. Following a global transposon mutagenesis screen of EAEC 042, the transcription factors, CRP and Fis, were identified as essential for transcription of the pet gene. Using both in vivo and in vitro techniques, we show that the pet promoter is co-dependent on CRP and Fis. We present a novel co-activation mechanism whereby CRP is placed at a non-optimal position for transcription initiation, creating dependence on Fis for full activation of pet. This study complements previous findings that establish Fis as a key virulence regulator in EAEC 042.
KW - Bacterial Toxins/biosynthesis
KW - Base Sequence
KW - Cyclic AMP Receptor Protein/metabolism
KW - DNA Transposable Elements
KW - Enterotoxins/biosynthesis
KW - Escherichia coli/genetics
KW - Escherichia coli Proteins/biosynthesis
KW - Factor For Inversion Stimulation Protein/metabolism
KW - Gene Expression Regulation, Bacterial
KW - Molecular Sequence Data
KW - Mutagenesis, Insertional
KW - Plasmids
KW - Serine Endopeptidases/biosynthesis
KW - Transcription, Genetic
UR - https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2011.07685.x
U2 - 10.1111/j.1365-2958.2011.07685.x
DO - 10.1111/j.1365-2958.2011.07685.x
M3 - Article
C2 - 21542864
SN - 0950-382X
VL - 81
SP - 179
EP - 191
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 1
ER -