Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review)

Richard A.J. Darby, Stephanie P. Cartwright, Marvin V. Dilworth, Roslyn M. Bill

Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

Abstract

Having decided on yeast as a production host, the choice of species is often the first question any researcher new to the field will ask. With over 500 known species of yeast to date, this could pose a significant challenge. However, in reality, only very few species of yeast have been employed as host organisms for the production of recombinant proteins. The two most widely used, Saccharomyces cerevisiae and Pichia pastoris, are compared and contrasted here.
Original languageEnglish
Title of host publicationRecombinant protein production in yeast
Subtitle of host publicationmethods and protocols
EditorsRoslyn M. Bill
PublisherHumana Press
Pages11-23
Number of pages13
ISBN (Electronic)978-1-61779-770-5
ISBN (Print)978-1-61779-769-9
DOIs
Publication statusPublished - 25 Feb 2012

Publication series

NameMethods in molecular biology
PublisherSpringer
Volume866
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029
NameSpringer protocols
PublisherSpringer

Bibliographical note

The original publication is available at www.springerlink.com

Keywords

  • yeast
  • host cell
  • recombinant protein production

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Dive into the research topics of 'Which yeast species shall I choose? Saccharomyces cerevisiae versus Pichia pastoris (review)'. Together they form a unique fingerprint.
  • Large-scale production of secreted proteins in Pichia pastoris

    Bora, N., 25 Feb 2012, Recombinant protein production in yeast. Bill, R. M. (ed.). Humana Press, p. 217-235 19 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

  • Optimising Pichia pastoris induction

    Bawa, Z. & Darby, R. A. J., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 181-190 10 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

  • Optimising yeast as a host for recombinant protein production (review)

    Bonander, N. & Bill, R. M., 25 Feb 2012, Recombinant protein production in yeast: methods and protocols. Bill, R. M. (ed.). Humana Press, p. 1-9 9 p. (Methods in molecular biology; vol. 866)(Springer protocols).

    Research output: Chapter in Book/Published conference outputChapter (peer-reviewed)peer-review

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